Human FGF7/KGF ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF7/KGF. 96wells/kit, with removable strips.
Human FGF7/KGF ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Product Name||Human FGF7/KGF ELISA Kit PicoKine™
See all KGF/FGF-7 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Kgf/FGF7 with <4pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Kgf/FGF7 tissue specificity: Epithelial cell.|
|Cite This Product||Human FGF7/KGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0820)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: C32-T194|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For FGF7 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2252|
|Species Of This Entry||Human|
|Protein Name||Fibroblast growth factor 7|
|Superfamily||heparin-binding growth factors family|
|Alternative Names||KGF/FGF-7|FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Biotinylation; Oxidation; Sulphation; Nitration; Glycosylation; Deacetylation; Acetylation; Dephosphorylation; Ubiquitination|
|Gene Location||15q21.2, on Chromasome 15, gene sequence: NC_000015.10 (49423242..49488775)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FGF7 (Source: Uniprot.org, NCBI)
|Protein Function||Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.|
|Tissue Specificity||Epithelial cell.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Hyperplasia; Carcinoma; Lung Injury; Inflammation; Prostatic Neoplasms; Fibrosis; Mucositis; Cell Transformation, Neoplastic; Mammary Neoplasms; Prostatic Hyperplasia; Malignant Neoplasm Of Prostate; Stomatitis; Benign Prostatic Hypertrophy; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Ulcer|
|Related Pathways||Cell Proliferation; Wound Healing; Cell Growth; Secretion; Regeneration; Epithelial Cell Proliferation; Cell Differentiation; Localization; Pathogenesis; Angiogenesis; Transport; Keratinocyte Proliferation; Lung Development; Cell Cycle; Cell Migration; Lung Morphogenesis; Cell Death; Epithelial Cell Differentiation; Gland Development; Keratinocyte Differentiation|
|Background||Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.|
|Scientific References||PMID: 2475908 by Finch P.W., et al. Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth.|
PMID: 1664700 by Aaronson S.A., et al. Keratinocyte growth factor. A fibroblast growth factor family member with unusual target cell specificity.
Kit Components And Assay QC Data Details Of Human FGF7/KGF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0820-CAP||96-well plate precoated with anti-Human KGF/FGF-7 antibody||1|
|EK0820-ST||lyophilized recombinant Human KGF/FGF-7 standard||10ng/tube|
|EK0820-DA||biotinylated anti-Human KGF/FGF-7 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0820 Human FGF7/KGF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human FGF7/KGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human FGF7/KGF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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