Human FGF7/KGF ELISA Kit PicoKine™

Human FGF7/KGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF7 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4pg/ml.

Product Info Summary

SKU: EK0820
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human FGF7/KGF ELISA Kit PicoKine™

See all KGF/FGF-7 products

SKU/Catalog Number

EK0820

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FGF7/KGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF7 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FGF7/KGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0820)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: C32-T194

Sensitivity

<4 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0820 is reactive to FGF7 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0820 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0820-CAP Anti-Human FGF7 Pre-coated 96-well strip microplate 1
EK0820-ST Human FGF7 Standard 2 vials, 10 ng/tube
EK0820-DA Human FGF7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.2 62.5 125 250 500 1000 2000
O.D.0.1250.1540.2330.3110.5661.1021.6722.371

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FGF7/KGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10026183797261764
Standard deviation7.516.1841.857.6620.3545.84
CV (%)7.5%6.2%5%7.9%7.8%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of KGF/FGF-7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1100869098935.726.1%
Sample 226129027627627510.253.7%
Sample 383791888090888531.43.5%
*number of samples for each test n=16.

Gene/Protein Information For FGF7 (Source: Uniprot.Org, NCBI)

Uniprot ID

P21781

Gene ID

2252

Gene Name

FGF7

Full Name

Fibroblast growth factor 7

Weight

22.509kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor) FGF7 HBGF-7, KGF fibroblast growth factor 7 fibroblast growth factor 7|FGF-7|heparin-binding growth factor 7|keratinocyte growth factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF7, check out the FGF7 Infographic

FGF7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human FGF7/KGF ELISA Kit PicoKine™

Question

Q: which procedure should I follow in order to thaw whole blood sample for KGF ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-06-28

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test KGF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-06-28

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Puri

Verified customer

Asked: 2018-01-31

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-01-31

Question

Q: if the enzyme conjugated KGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the KGF antigen?

W. Miller

Verified customer

Asked: 2017-05-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-05-23

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement KGF using the EK0820 Human KGF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-04-17

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2016-04-17

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