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MKI67 antibody (M00254-8) performs well in Flow Cytometry, IF, IHC, ICC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of Jurkat cells using anti-Ki67 antibody (M00254-8).
Overlay histogram showing Jurkat cells stained with M00254-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (M00254-8, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with M00254-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (M00254-8, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Ki67 Antibody (monoclonal, 5E12)
MKI67 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC |
Tested Applications
MKI67 antibody was positively detected by IHC in:
human tonsil tissue, human cervical cancer tissue, human esophageal squamous carcinoma tissue, human liver tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MKI67 antibody was positively detected by ICC/IF in:
A431 cell
MKI67 antibody was positively detected by IF in:
human tonsil tissue
MKI67 antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Ki67 Antibody (monoclonal, 5E12)
View all MKI67 Antibodies
View all MKI67 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5E12
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
358.7 kDa
Background of MKI67
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Cite This Product
Anti-Ki67 Antibody (monoclonal, 5E12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-8)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
90%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for MKI67 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MKI67 antibody
Immunofluorescence (IF) protocol for MKI67 antibody
Flow Cytometry (FC) protocol for MKI67 antibody
Publications
Application:
Species:
The oocyte-enriched metabolite serotonin alleviates cellular senescence and aging phenotypes in the mouse
Published: 2026-06-16
Journal: EMBO JOURNAL
Application: IF
Impact Factor: 8.40
Species: Human
PMID: 42303757
AHR regulates liver enlargement and regeneration through the YAP signaling pathway
Published: 2024-09-02
Journal: Heliyon
Application: IHC
Impact Factor: 3.40
Species: Mouse
PMID: 39296106
Understanding the dynamics of TKI-induced changes in the tumor immune microenvironment for improved therapeutic effect
Published: 2024-06-01
Journal: Journal for ImmunoTherapy of Cancer
Application: IF
Impact Factor: 10.30
Species: Human
PMID: 38908857
Brigatinib, a newly discovered AXL inhibitor, suppresses AXL-mediated acquired resistance to osimertinib in EGFR-mutated non-small cell lung cancer
Published: 2024-03-04
Journal: ACTA PHARMACOLOGICA SINICA
Application: IF
Impact Factor: 6.90
Species: Human
PMID: 38438582
Mitochondrial dysfunction affects hepatic immune and metabolic remodeling in patients with hepatitis B virus-related acute-on-chronic liver failure
Published: 2024-02-28
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IF
Impact Factor: 4.30
Species: Human
PMID: 38516248
A cell transmembrane peptide chimeric M(27-39)-HTPP targeted therapy for hepatocellular carcinoma
Published: 2023-04-29
Journal: iScience
Application: IF
Impact Factor: 5.80
Species: Mouse
PMID: 37234089
Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling
Published: 2022-02-23
Journal: Communications Biology
Application: IF
Impact Factor: 6.55
Species: Human
PMID: 35197546
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Product Promise
We promise Anti-Ki67 Antibody (monoclonal, 5E12) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00254-8
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026