Anti-Ki67/MKI67 Antibody

Ki67/MKI67 antibody

Boster Bio Anti-Ki67/MKI67 Antibody catalog # PA1918. Tested in WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PA1918
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Ki67/MKI67 Antibody

View all Ki67/MKI67 Antibodies

SKU/Catalog Number

PA1918

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ki67/MKI67 Antibody catalog # PA1918. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ki67/MKI67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1918)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67 (3234-3256aa KKAEDNVCVKKIRTRSHRDSEDI).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1918 is reactive to MKI67 in Human

Applications

PA1918 is guaranteed for WB Boster Guarantee

Background of Ki67/MKI67

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, Schonk et al. (1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)

Uniprot ID

P46013

Gene ID

4288

Gene Name

MKI67

Full Name

Proliferation marker protein Ki-67

Weight

358.694kDa

Alternative Names

antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MKI67, check out the MKI67 Infographic

MKI67 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1918 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
PA1918 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1

The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex

715: [email protected] in combination with radiotherapy induces the apoptosis of HepG2 cells by modulating the expression of apoptosis-related proteins

Mutant p53 induces EZH2 expression and promotes epithelial?mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing

Solamargine triggers hepatoma cell death through apoptosis

WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

HER2-siRNA delivered by EGFR-specific single chain antibody inhibits NSCLC cell proliferation and tumor growth

Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

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PA1918
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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