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Product Info Summary
| SKU: | MA1077 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CDK1 Antibody (Monoclonal, A17)
SKU/Catalog Number
MA1077
BM0027 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDK1 Antibody (Monoclonal, A17) catalog # MA1077. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK1 Antibody (Monoclonal, A17) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1077)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
A17
Isotype
Mouse IgG2a
Immunogen
C-terminal two-thirds of Xenopus CDK1 expressed in E.coli.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1077 is reactive to Cdk1 in Chicken, Human, Mouse
Observed Molecular Weight
18 kDa
Calculated molecular weight
34.1 kDa
Background of Cdk1
P34 (cdc2), also known as cell division cycle (CDC2), or cyclin-dependent kinase 1 (CDK1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1077 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 0.5-1μg/ml, Human, chicken, mouse
Validation Images & Assay Conditions
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Boster Kit Box
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Real-time qPCR analysis of As-cdc20 and As-cdc23 expression levels at different developmental stages of A. sinica . Significant differences at different development stages ( P < 0.05) were analyzed by multi-factor analysis of variance (MANOVA) and indicated by lowercase letters (a, b and c).
Index in PubMed under a CC BY license. PMID: 27991546
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The results of prokaryotic expression and antibody detection of As -CDC20 protein. (a) Expression analysis of the recombinant As -CDC20 protein. (b) Detection of the solubility of the recombinant As -CDC20 protein. (c) Western blotting analysis of the specific binding of the purified recombinant protein with antiserum antibodies. The full-length gels are presented in .
Index in PubMed under a CC BY license. PMID: 27991546
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The results of prokaryotic expression and antibody detection of As -CDC23 protein. (a) Expression of the recombinant As -CDC23 protein. (b) Detection of the solubility of the recombinant As -CDC23 protein. (c) Purification of the recombinant As -CDC23 protein. (d) Western blotting analysis of the specific binding of the purified recombinant protein with antiserum antibodies. The full-length gels are presented in .
Index in PubMed under a CC BY license. PMID: 27991546
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Western blotting analysis of As -CDC20, As -CDH1, As -CDC23, As -CDC14B, As -Securin, As -Cyclin B, As -CDK1 and As -Geminin. (a) Western blotting showing the expression of proteins at different developmental stages of Artemia sinica . (b) Values are expressed as arbitrary units of relative value. Significant differences ( P < 0.05) were analyzed by MANOVA and indicated by lowercase letters (a,b,c and d). The images are cropped, and the full-length blots are presented in .
Index in PubMed under a CC BY license. PMID: 27991546
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Immunolocalization analysis of As -CDC23 at the embryo and sub-adult stages in A. sinica . A-A3: 0 h; B-B3: 15 h; C-C3: 3d; ( A–C ) represent anti-CDC23-label; A1-C1: single-labeling with DAPI; A2-C2 represent the image overlay of the samples dual-labeled with polyclonal anti-CDC23 and DAPI. A3-C3 represent control group.
Index in PubMed under a CC BY license. PMID: 27991546
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The double-labeled immunofluorescence analysis of As -CDC20 and As -CDH1 in A. sinica . A–A3: gastrula stage; B-B3: embryonic stage; C-C3: metanauplius stage; ( A,B and C ) represent single-labeling with anti-CDC20; A1-C1: single-labeling with anti-CDH1; A2-C2 represent the image overlay of dual-labeled samples. A3–C3 represent the control group.
Index in PubMed under a CC BY license. PMID: 27991546
Specific Publications For Anti-CDK1 Antibody (Monoclonal, A17) (MA1077)
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Customer Q&As
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4 Customer Q&As for Anti-CDK1 Antibody (Monoclonal, A17)
Question
We ordered your anti-CDK1 antibody (Monoclonal, A17) for WB on cervix carcinoma erythroleukemia last year. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
I have checked the website and datasheets of our anti-CDK1 antibody (Monoclonal, A17) and I see that MA1077 has been validated on human in both WB and IHC. Thus MA1077 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-18
Question
We have observed staining in mouse cervix carcinoma. What should we do? Is anti-CDK1 antibody (Monoclonal, A17) supposed to stain cervix carcinoma positively?
W. Mangal
Verified customer
Asked: 2018-11-26
Answer
Based on literature cervix carcinoma does express CDK1. Based on Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-26
Question
Our lab were satisfied with the WB result of your anti-CDK1 antibody (Monoclonal, A17). However we have seen positive staining in mammary cancer nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
R. Thomas
Verified customer
Asked: 2013-11-21
Answer
From literature, mammary cancer does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-11-21
Question
We are currently using anti-CDK1 antibody (Monoclonal, A17) MA1077 for chicken tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse. Is it likely that the antibody can work on feline tissues as well?
M. Miller
Verified customer
Asked: 2013-08-13
Answer
The anti-CDK1 antibody (Monoclonal, A17) (MA1077) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-13
