Anti-CDK1 Antibody (Monoclonal, A17)

Boster Bio Anti-CDK1 Antibody (Monoclonal, A17) catalog # MA1077. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse. Cited in 12 publication(s).

Product Info Summary

SKU: MA1077
Size: 100μg/vial
Reactive Species: Chicken, Human, Mouse
Host: Mouse
Application: IHC, WB

Product Name

Anti-CDK1 Antibody (Monoclonal, A17)

See all CDC2/CDK1 products

SKU/Catalog Number

MA1077

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK1 Antibody (Monoclonal, A17) catalog # MA1077. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK1 Antibody (Monoclonal, A17) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1077)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

A17

Isotype

Mouse IgG2a

Immunogen

C-terminal two-thirds of Xenopus CDK1 expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1077 is reactive to Cdk1 in Chicken, Human, Mouse

Applications

MA1077 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 0.5-1μg/ml, Human, chicken, mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cdk1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P39951

Gene ID

54237

Gene Name

Cdk1

Full Name

Cyclin-dependent kinase 1

Weight

34.135kDa

Superfamily

protein kinase superfamily

Alternative Names

CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2 Cdk1|Cdc2, Cdc2a|cyclin-dependent kinase 1|cyclin-dependent kinase 1|Cell division cycle control protein 2|cell division control protein 2 homolog|cell division cycle 2 homolog A|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cdk1, check out the Cdk1 Infographic

Cdk1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cdk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1077 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Blakemore D,Vilaplana-Lopera N,Almaghrabi R,Gonzalez E,Moya M,Ward C,Murphy G,Gambus A,Petermann E,Stewart GS,García P.MYBL2 and ATM suppress replication stress in pluripotent stem cells.EMBO Rep.2021 Mar 28:e51120.doi:10.15252/embr.202051120.Epub ahead of print.PMID:33779025.
Species: Mouse
MA1077 usage in article: APP:WB, SAMPLE:ESCS, DILUTION:NA

Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
MA1077 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:500

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Human enterovirus 68 interferes with the host cell cycle to facilitate viral production

Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production

Ren Y, Wang Q, Shi L, Yue W, Zhang C, Lei F. Anim Reprod Sci. 2011 Feb;123(3-4):187-91. Doi: 10.1016/J.Anireprosci.2011.01.002. Epub 2011 Jan 14. Effects Of Maternal And Dietary Selenium (Se-Enriched Yeast) On The Expression Of P34(Cdc2) And Cycli...

Yang C, Yang J, Sun M, Yan J, Meng X, Ma T. Iubmb Life. 2013 May;65(5):435-44. Doi: 10.1002/Iub.1141. Epub 2013 Feb 26. Alantolactone Inhibits Growth Of K562/Adriamycin Cells By Downregulating Bcr/Abl And P-Glycoprotein Expression.

Zeng Zj, Xiang Sg, Xue Ww, Li Hd, Ma N, Ren Zj, Xu Zj, Jiao Ch, Wang Cy, Hu Wx. Biomed Pharmacother. 2014 Sep;68(7):887-92. Doi: 10.1016/J.Biopha.2014.07.018. Epub 2014 Jul 28. The Cell Death And Dna Damages Caused By The Tet-On Regulating Hsv-Tk/...

Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.

Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...

Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

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4 Customer Q&As for Anti-CDK1 Antibody (Monoclonal, A17)

Question

We ordered your anti-CDK1 antibody (Monoclonal, A17) for WB on cervix carcinoma erythroleukemia last year. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

I have checked the website and datasheets of our anti-CDK1 antibody (Monoclonal, A17) and I see that MA1077 has been validated on human in both WB and IHC. Thus MA1077 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-18

Question

We have observed staining in mouse cervix carcinoma. What should we do? Is anti-CDK1 antibody (Monoclonal, A17) supposed to stain cervix carcinoma positively?

W. Mangal

Verified customer

Asked: 2018-11-26

Answer

Based on literature cervix carcinoma does express CDK1. Based on Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-11-26

Question

Our lab were satisfied with the WB result of your anti-CDK1 antibody (Monoclonal, A17). However we have seen positive staining in mammary cancer nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?

R. Thomas

Verified customer

Asked: 2013-11-21

Answer

From literature, mammary cancer does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-11-21

Question

We are currently using anti-CDK1 antibody (Monoclonal, A17) MA1077 for chicken tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse. Is it likely that the antibody can work on feline tissues as well?

M. Miller

Verified customer

Asked: 2013-08-13

Answer

The anti-CDK1 antibody (Monoclonal, A17) (MA1077) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-13

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