Anti-Ki67/MKI67 Antibody Picoband™

Ki67/MKI67 antibody

Boster Bio Anti-Ki67/MKI67 Antibody Picoband™ catalog # PB9026. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB9026
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-Ki67/MKI67 Antibody Picoband™

View all Ki67/MKI67 Antibodies

SKU/Catalog Number

PB9026

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ki67/MKI67 Antibody Picoband™ catalog # PB9026. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ki67/MKI67 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9026)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9026 is reactive to MKI67 in Human

Applications

PB9026 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Background of Ki67/MKI67

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)

Uniprot ID

P46013

Gene ID

4288

Gene Name

MKI67

Full Name

Proliferation marker protein Ki-67

Weight

358.694kDa

Alternative Names

antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MKI67, check out the MKI67 Infographic

MKI67 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9026 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients

Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells in vitro and in vivo by regulating the ERK signaling pathway

PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation

Elevated CREPT Expression Enhances the Progression of Salivary Gland Adenoid Cystic Carcinoma

Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer

R2: Identification of renal potential progenitor/stem cells that participate in the renal regeneration processes of kidney allograft fibrosis

Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia

LRRC4 mediates the formation of circular RNA CD44 to inhibit GBM cell proliferation

Effects of co-culturing BMSCs and auricular chondrocytes on the elastic modulus and hypertrophy of tissue engineered cartilage
Species: Mouse

LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes
Species: Human

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1 Reviews For Anti-Ki67/MKI67 Antibody Picoband™

0

Immunocytochemistry forAnti-P53/TP53 Antibody

Excellent

Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Verified customer

Submitted

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3 Customer Q&As for Anti-Ki67/MKI67 Antibody Picoband™

Question

We are currently using anti-Ki67/MKI67 antibody PB9026 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

The anti-Ki67/MKI67 antibody (PB9026) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-17

Question

Is PB9026 suitable to stain ki67 with mouse and rat sample IHC-P sections?

Verified customer

Asked: 2019-05-15

Answer

The Anti-Ki67/MKI67 Antibody Picoband™ (PB9026) is only suitable for human samples.

Boster Scientific Support

Answered: 2019-05-16

Question

Has PB9026 been tested on mouse and rat samples for IHC-P?

Verified Customer

Verified customer

Asked: 2017-08-25

Answer

PB9026 is only suitable for human samples.

Boster Scientific Support

Answered: 2017-08-25

Size

Conjugation

Total: $315

SKU:PB9026

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PB9026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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