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MKI67 antibody (PB9026) performs well in IF, IHC, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 34 Publications

pb9026 ki67 primary antibodies if testing 3

IF analysis of Ki67 using anti-Ki67 antibody (PB9026).
Ki67 was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Ki67/MKI67 Antibody Picoband®

Name: Anti-Ki67/MKI67 Antibody Picoband®
Brand: Boster Bio
SKU: PB9026
Price: 370 USD
Availability: InStock
Rating: 100 (3 reviews)

MKI67 antibody

34 Publications|

3 Reviews

|Submit a Review

Reactive Species	Host	Application
Human	Rabbit	IF, IHC, ICC, WB

Tested Applications

MKI67 antibody was positively detected by WB in:

HELA Whole Cell, MCF-7 Whole Cell, COLO320 Whole Cell, HEPG2 Whole Cell, SKOV Whole Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MKI67 antibody was positively detected by IHC in:

human tonsil tissue, human lung cancer tissu, human breast cancer tissue, nude mouse tumor tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MKI67 antibody was positively detected by ICC in:

Hela cell

MKI67 antibody was positively detected by ICC/IF in:

Hela cell, SiHa cell

MKI67 antibody was positively detected by IF in:

human colon organoid tissue, nude mouse tumor tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Monkey

Predicted

Product Information

Product Name

Anti-Ki67/MKI67 Antibody Picoband®
View all MKI67 Antibodies

Form

Lyophilized

Uniprot

P46013

Gene ID

4288

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

358 kDa

Calculated Molecular Weight

358.7 kDa

Background of MKI67

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Cite This Product

Anti-Ki67/MKI67 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9026)

Protocols

Western Blot protocol for MKI67 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MKI67 antibody

Review Protocol

Immunocytochemistry (ICC) protocol for MKI67 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MKI67 antibody

Review Protocol

Immunofluorescence (IF) protocol for MKI67 antibody

Review Protocol

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Published: 2026-08-26

Journal: CLINICAL AND EXPERIMENTAL MEDICINE

Application: IHC

Impact Factor: 4.50

Species: Mouse

SKU	PB9026
Application	Western Blot
Sample	Nude mouse tumor tissue
Sample Processing Description	Paraffin-embedded tumor tissue sections.
Primary Antibody	Ki67/MKI67 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary	This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

SKU	PB9026
Application	Immunofluorenscence
Sample	Cells in nude mouse tumor tissue
Sample Processing Description	Sections of nude mouse tumor tissues fixed and embedded under different treatment conditions (Scale Bar = 100 μm)
Primary Antibody	Anti-Ki67/MKI67 Antibody Picoband®
Primary Incubation	1:200, overnight at 4 ℃
Blocking Agent	Goat serum
Secondary Antibody	DyLight 488-conjugated goat anti-rabbit antibody.
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Fluorescence microscope
Results Summary	The antibodies used in the experiment demonstrated good sensitivity and high cost-effectiveness, providing strong support for the smooth progress of the study.

SKU	PB9026
Application	Immunohistochemistry (Paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Human tonsil
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	37°C for 30 minutes
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	2ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	4°C overnight

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Anti-Ki67/MKI67 Antibody Picoband®

MKI67 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

piR‑164552 promotes malignant progression in head and neck squamous cell carcinoma via the RBM4/EIF4E2 signaling axis: Insights from multi‑omics and functional analyses

Scaffold-assisted assembly of cell-laden microgels to engineer permeable living constructs for vascularized adipose tissue regeneration

Single-Cell Transcriptomic Profiling of Human Blood Vessel Organoids Exposed to 6-Chloronicotinic Acid

Clinical significance of immunocyte- and ferroptosis-related markers for the prognosis of patients with tenosynovial giant cell tumor: A cross-sectional study

NOTCH4 downregulation repolarizes tumor-associated macrophages and enhances the sorafenib sensitivity of colorectal cancer cells

Lycorine Suppresses DPP3-Mediated Nrf2 Signaling, Thereby Reducing SLC7A11 Levels to Trigger Ferroptosis in Colorectal Cancer Cells

Echinacoside improves antioxidant responses and angiogenesis through Parkin-MFN2-mediated mitophagy to promote diabetic wound healing

GATA3-NLRP1 Axis Inhibits Hepatocellular Carcinoma Progression Through the Hedgehog Signaling

Bioinformatics and In Vitro/In Vivo Experiments Identify MAD2L1 as an m6A-Associated Biomarker Promoting Oral Squamous Cell Carcinoma Progression

Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells

Cascade pH-responsive multi-drug delivery nanosystem actuates PANoptosis of triple-negative breast cancer

Development of an Effective Drug Carrier for Targeted Therapy in Lung Adenocarcinoma Using ROS-responsive Micelles

Senecavirus a can replicate in apical-out porcine intestinal organoids and induce stress granules and innate immune response

KMT2D/ZNF460-induced COL9A1-mediated extracellular matrix stiffness maintains the cancer stem cell pool to promote colorectal cancer progression

Efficacy assessment of glycyrrhetinic acid-modified liposomes loaded with doxorubicin hydrochloride and cucurbitine B for synergistic treatment of hepatocellular carcinoma

TFAP2A downregulation mediates tumor-suppressive effect of miR-8072 in triple-negative breast cancer via inhibiting SNAI1 transcription

PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer

miR-134-3p driven by anisomycin impairs ovarian cancer stem cell activity through inhibiting GPR137 expression

Efficient intervention for pulmonary fibrosis via mitochondrial transfer promoted by mitochondrial biogenesis

Anisomycin inhibits the activity of human ovarian cancer stem cells via regulating antisense RNA NCPB2-AS2/MEK/ERK/STAT3 signaling

Expression and Clinical Significance of Ki67 and SOX2 in Colorectal Cancer

LRRC4 mediates the formation of circular RNA CD44 to inhibit GBM cell proliferation

Elevated CREPT Expression Enhances the Progression of Salivary Gland Adenoid Cystic Carcinoma

Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia

Circ_0001955 Acts as a miR-646 Sponge to Promote the Proliferation, Metastasis and Angiogenesis of Hepatocellular Carcinoma

Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells in vitro and in vivo by regulating the ERK signaling pathway

Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer

Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients

Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma

miR-494-3p Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by PTEN/AKT Signaling in Human Glioblastoma Cells

R2: Identification of renal potential progenitor/stem cells that participate in the renal regeneration processes of kidney allograft fibrosis

PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation

Review

Have you used Anti-Ki67/MKI67 Antibody Picoband®?

3 Reviews For Anti-Ki67/MKI67 Antibody Picoband®

In IHC using Ki67 antibody (Cat# PB9026), strong and specific nuclear staining was observed in nude mouse tumor tissue, confirming high Ki67 expression and active cell proliferation.

BOSTER’s Ki-67 antibody can effectively mark the proliferative activity of tumor cells. After treatment, a significant decrease in Ki-67 expression in tumor tissues can be clearly observed, indicating that the proliferative activity of tumor cells is mark

Immunocytochemistry forAnti-P53/TP53 Antibody

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