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Product Info Summary
| SKU: | A01850 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-KIR2.1 KCNJ2 Antibody
SKU/Catalog Number
A01850
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KIR2.1 KCNJ2 Antibody catalog # A01850. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KIR2.1 KCNJ2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01850)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KCNJ2. AA range:81-130
Reactive Species
A01850 is reactive to KCNJ2 in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01850 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using KIR2.1 Polyclonal Antibody diluted at 1:500
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KCNJ2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-KIR2.1 KCNJ2 Antibody (A01850)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-KIR2.1 KCNJ2 Antibody
Question
Is this A01850 anti-KIR2.1 antibody reactive to the isotypes of KCNJ2?
K. Mangal
Verified customer
Asked: 2020-01-24
Answer
The immunogen of A01850 anti-KIR2.1 antibody is Synthesized peptide derived from the Internal region of human KIR2.1. at AA rangle: 50-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-24
Question
I see that the anti-KIR2.1 antibody A01850 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-07
Question
Will anti-KIR2.1 antibody A01850 work for WB with fetal brain heart?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
According to the expression profile of fetal brain heart, KCNJ2 is highly expressed in fetal brain heart. So, it is likely that anti-KIR2.1 antibody A01850 will work for WB with fetal brain heart.
Boster Scientific Support
Answered: 2018-08-22
Question
Is there a BSA free version of anti-KIR2.1 antibody A01850 available?
A. Banerjee
Verified customer
Asked: 2017-11-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KIR2.1 antibody A01850 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-17
Question
Will A01850 anti-KIR2.1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Anderson
Verified customer
Asked: 2017-10-02
Answer
It shows on the product datasheet, A01850 anti-KIR2.1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-02
