Product Info Summary
SKU: | EK1372 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human ADAMTS4 ELISA Kit PicoKine®
SKU/Catalog Number
EK1372
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ADAMTS4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAMTS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ADAMTS4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1372)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: F213-C685
Sensitivity
<20 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1372 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1372 is reactive to ADAMTS4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1372 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ADAMTS4
ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1372-CAP | Anti-Human ADAMTS4 Pre-coated 96-well strip microplate | 1 |
EK1372-ST | Human ADAMTS4 Standard | 2 vials, 40 ng/tube |
EK1372-DA | Human ADAMTS4 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.025 | 0.068 | 0.123 | 0.224 | 0.435 | 0.710 | 1.194 | 1.815 |
Data Example Images
Click image to see more details
Human ADAMTS4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ADAMTS4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 625 pg/ml..
Some articles we found to cite concentrations of ADAMTS4 in samples: 20625753 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ADAMTS4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1127 | 4290 | 29063 | 1210 | 4672 | 29313 |
Standard deviation | 46.2 | 184.47 | 1627.52 | 71.39 | 200.89 | 2081.22 |
CV (%) | 4.1% | 4.3% | 5.6% | 5.9% | 4.3% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ADAMTS4 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1127 | 1186 | 1062 | 1229 | 1151 | 62.86 | 5.4% |
Sample 2 | 4290 | 4643 | 4014 | 4511 | 4364 | 238.44 | 5.4% |
Sample 3 | 29063 | 29288 | 30434 | 26599 | 28846 | 1397.6 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For ADAMTS4 (Source: Uniprot.Org, NCBI)
Gene Name
ADAMTS4
Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 4
Weight
90.197kDa
Alternative Names
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS2; ADAMTS-2; ADAMTS4; ADAM-TS4; ADAMTS-4; ADMP-1; ADMP-1EC 3.4.24.82; Aggrecanase 1; aggrecanase-1; EC 3.4.24; KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4 ADAMTS4 ADAMTS-2, ADAMTS-4, ADMP-1 ADAM metallopeptidase with thrombospondin type 1 motif 4 A disintegrin and metalloproteinase with thrombospondin motifs 4|ADAM-TS 4|ADAM-TS4|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4|aggrecanase-1|epididymis secretory sperm binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ADAMTS4, check out the ADAMTS4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ADAMTS4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ADAMTS4 ELISA Kit PicoKine® (EK1372)
Hello CJ!
EK1372 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Galectin-1 Couples Glycobiology to Inflammation in Osteoarthritis through the Activation of an NF-κB–Regulated Gene Network
Galectin-3 Induces a Pro-degradative/inflammatory Gene Signature in Human Chondrocytes, Teaming Up with Galectin-1 in Osteoarthritis Pathogenesis
Sodium butyrate protects against oxidative stress in human nucleus pulposus cells via elevating PPARγ-regulated Klotho expression
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human ADAMTS4 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human ADAMTS4 Picokine® ELISA Kit (Catalog # EK1372)?
Verified Customer
Verified customer
Asked: 2020-10-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of ADAMTS4 causing disruption of its protein structure. ADAMTS4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the ADAMTS4 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis ADAMTS4 using the EK1372 Human ADAMTS4 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-10-29
Question
Q: What is the optimal O.D. value for ADAMTS4 ELISA kit? I performed your ADAMTS4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ADAMTS4 even though the O.D. values are not very high?
H. Smith
Verified customer
Asked: 2019-08-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ADAMTS4 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of ADAMTS4 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2019-08-11
Question
Q: if the enzyme conjugated ADAMTS4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ADAMTS4 antigen?
V. Mangal
Verified customer
Asked: 2019-08-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Evans
Verified customer
Asked: 2019-07-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-07-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of ADAMTS4 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-01-12
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ADAMTS4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-12
Question
I am interested in your EK1372 ADAMTS4 ELISA kit. I would like to use this kit with Rheumatoid arthritis, Osteoarthritis patient, so may I know if you have any information or citation or customer feedback show that the kit could be used for these kinds of patient samples?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
Our lab technicians haven't
Boster Scientific Support
Answered: 2018-09-21
Question
Q: how to proceed with the analysis of ELISA data? I have obtained ADAMTS4 level in serum.
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-22
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-12-18
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-12-18