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Product Info Summary
| SKU: | A16260 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-KLHL11 Monoclonal Antibody
SKU/Catalog Number
A16260
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KLHL11 Monoclonal Antibody catalog # A16260. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLHL11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16260)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1B9C1
Isotype
IgG
Immunogen
Purified recombinant fragment of human KLHL11 expressed in E. Coli.
Reactive Species
A16260 is reactive to KLHL11 in Human
Calculated molecular weight
80.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16260 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis using KLHL11 Monoclonal Antibody against HeLa (1) and MCF-7 (2) cell lysate.
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Immunohistochemistry analysis of paraffin-embedded liver cancer (right) and colon cancer tissues (left) with DAB staining using KLHL11 Monoclonal Antibody.
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Immunofluorescence analysis of NIH/3T3 cells using KLHL11 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Specific Publications For Anti-KLHL11 Monoclonal Antibody (A16260)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-KLHL11 Monoclonal Antibody
Question
Is there a BSA free version of anti-KLHL11 Monoclonal antibody A16260 available?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KLHL11 Monoclonal antibody A16260 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-09
Question
Can you help my question with product A16260, anti-KLHL11 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16260 anti-KLHL11 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-29
Question
We are currently using anti-KLHL11 Monoclonal antibody A16260 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-KLHL11 Monoclonal antibody (A16260) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
Question
I am interested in to test anti-KLHL11 Monoclonal antibody A16260 on human sperm for research purposes, then I may be interested in using anti-KLHL11 Monoclonal antibody A16260 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The products we sell, including anti-KLHL11 Monoclonal antibody A16260, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-26
Question
See attached the WB image, lot number and protocol we used for sperm using anti-KLHL11 Monoclonal antibody A16260. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-18
Question
I see that the anti-KLHL11 Monoclonal antibody A16260 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-05-12
Question
Will A16260 anti-KLHL11 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Singh
Verified customer
Asked: 2014-02-27
Answer
It shows on the product datasheet, A16260 anti-KLHL11 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-27
