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- Table of Contents
LMNA antibody (M00438-6) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6).
Lamin A+C/LMNA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Lamin A+C/LMNA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12)
LMNA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, WB |
Tested Applications
LMNA antibody was positively detected by WB in:
human placenta tissue, human Hela whole cell, human A431 whole cell, rat lung tissue, mouse lung tissue, mouse small intestine tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LMNA antibody was positively detected by IHC in:
human laryngeal squamous cell carcinoma tissue, human ovarian cancer tissue, human bladder epithelial carcinoma tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 µg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 µg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12)
View all LMNA Antibodies
View all LMNA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5F3C12
Isotype
IgG2b
Immunogen
E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
74 kDa
Calculated Molecular Weight
74.1 kDa
Background of LMNA
Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
Cite This Product
Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00438-6)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
98%
High Homology
Rabbit
98%
High Homology
Sheep
97%
High Homology
Cow
96%
High Homology
Rat
93%
High Homology
Mouse
92%
High Homology
Zebrafish
52%
Low homology
Drosophila
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LMNA antibody
Immunohistochemistry (IHC) protocol for LMNA antibody
Publications
Application:
Species:
Polysaccharides from Polygonatum kingianum Collett & Hemsl ameliorated fatigue by regulating NRF2/HO-1/NQO1 and AMPK/PGC-1α/TFAM signaling pathways, and gut microbiota
Published: 2024-04-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 7.70
Species: Mouse
PMID: 38593898
Review
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Product Promise
We promise Anti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00438-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026