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Product Info Summary
| SKU: | PB9280 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Lamin A+C/LMNA Antibody Picoband™
SKU/Catalog Number
PB9280
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lamin A+C/LMNA Antibody Picoband™ catalog # PB9280. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin A+C/LMNA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9280)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
PB9280 is reactive to LMNA in Human, Mouse, Rat
Applications
PB9280 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Background of LMNA
Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Anti-Lamin A Picoband antibody, PB9280, Western blotting
All lanes: Anti Lamin A (PB9280) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted bind size: 74KD
Observed bind size: 74KD
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Anti-Lamin A Picoband antibody, PB9280, IHC(P)
IHC(P): Mouse Intestine Tissue
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Anti-Lamin A Picoband antibody, PB9280, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Lamin A Picoband antibody, PB9280, IHC(P)
IHC(P): Rat Intestine Tissue
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Figure 5. IF analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280).
Lamin A/C was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 6. IF analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280).
Lamin A/C was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 7. Flow Cytometry analysis of THP-1 cells using anti-Lamin A/C antibody (PB9280).
Overlay histogram showing THP-1 cells stained with PB9280 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lamin A/C Antibody (PB9280,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LMNA (Source: Uniprot.Org, NCBI)
Uniprot ID
P02545
Gene ID
4000
Gene Name
LMNA
Full Name
Prelamin-A/C
Weight
74.139kDa
Superfamily
intermediate filament family
Alternative Names
CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LMNA, check out the LMNA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LMNA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Lamin A+C/LMNA Antibody Picoband™ (PB9280)
PB9280 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Loss of lamin A but not lamin C expression in epithelial ovarian cancer cells is associated with metastasis and poor prognosis
Li D,Liu J,Yang C,Tian Y,Yin C,Hu L,Chen Z,Zhao F,Zhang R,Lu A,Zhang G,Qian A.Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.Theranostics.2021 Mar 20;11(11):5585-5604.doi:10.7150/thno.54477. PMID:33859765;PMCID:PMC8039942.
Species: Human,Mouse
PB9280 usage in article: APP:WB, SAMPLE:HFOB1.19 CELL AND FEMUR TISSUE, DILUTION: 1:1000
714: Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells
Yang Lm, Liu Xl, Zhu Qt, Zhang Y, Xi Tf, Hu J, He Cf, Jiang L. J Biomed Mater Res B Appl Biomater. 2011 Jan;96(1):25-33. Doi: 10.1002/Jbm.B.31719. Human Peripheral Nerve-Derived Scaffold For Tissue-Engineered Nerve Grafts: Histology And Biocompati...
Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Lamin A+C/LMNA Antibody Picoband™
Question
My question regards to test anti-Lamin A+C/LMNA antibody PB9280 on mouse mucosa of stomach for research purposes, then I may be interested in using anti-Lamin A+C/LMNA antibody PB9280 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The products we sell, including anti-Lamin A+C/LMNA antibody PB9280, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-27
Question
See below the WB image, lot number and protocol we used for mucosa of stomach using anti-Lamin A+C/LMNA antibody PB9280. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
Would PB9280 anti-Lamin A+C/LMNA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
You can see on the product datasheet, PB9280 anti-Lamin A+C/LMNA antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-04
Question
Do you have a BSA free version of anti-Lamin A+C/LMNA antibody PB9280 available?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Lamin A+C/LMNA antibody PB9280 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-17
Question
My colleagues were satisfied with the WB result of your anti-Lamin A+C/LMNA antibody. However we have seen positive staining in corpus callosum nucleus. using this antibody. Is that expected? Could you tell me where is LMNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
According to literature, corpus callosum does express LMNA. Generally LMNA expresses in nucleus., isoform c: nucleus speckle. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2019-07-18
Question
We are currently using anti-Lamin A+C/LMNA antibody PB9280 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The anti-Lamin A+C/LMNA antibody (PB9280) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
Question
Is a blocking peptide available for product anti-Lamin A+C/LMNA antibody (PB9280)?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
We do provide the blocking peptide for product anti-Lamin A+C/LMNA antibody (PB9280). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-19
Question
We bought anti-Lamin A+C/LMNA antibody for WB on liver in a previous experiment. I am using rat, and I plan to use the antibody for IHC next. We want examining liver as well as lung skin in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
I have checked the website and datasheets of our anti-Lamin A+C/LMNA antibody and it seems that PB9280 has been validated on rat in both WB and IHC. Thus PB9280 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-27
Question
Is this PB9280 anti-Lamin A+C/LMNA antibody reactive to the isotypes of LMNA?
A. Jones
Verified customer
Asked: 2018-04-12
Answer
The immunogen of PB9280 anti-Lamin A+C/LMNA antibody is E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-12
Question
Can you help my question with product PB9280, anti-Lamin A+C/LMNA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9280 anti-Lamin A+C/LMNA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of stomach using anti-Lamin A+C/LMNA antibody PB9280. Let me know if you need anything else.
J. Krishna
Verified customer
Asked: 2017-12-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-28
Question
We are interested in using your anti-Lamin A+C/LMNA antibody for muscle organ development studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.
G. Krishna
Verified customer
Asked: 2017-03-16
Answer
I appreciate your inquiry. This PB9280 anti-Lamin A+C/LMNA antibody is validated on human placenta tissue, tissue lysate, skov whole cell lysate, sw620 whole cell lysate, colo320 whole cell lysate, hela whole cell lysate, 293t whole cell lysate, a549 whole cell lysate, mouse intestine tissue, mammary cancer tissue, rat intestine tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-03-16
Question
We have been able to see staining in mouse renal cell carcinoma. Do you have any suggestions? Is anti-Lamin A+C/LMNA antibody supposed to stain renal cell carcinoma positively?
N. Johnson
Verified customer
Asked: 2015-11-23
Answer
Based on literature renal cell carcinoma does express LMNA. Based on Uniprot.org, LMNA is expressed in mucosa of stomach, corpus callosum, kidney, lung skin, ovarian carcinoma, colon, renal cell carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2015-11-23
Question
I see that the anti-Lamin A+C/LMNA antibody PB9280 works with WB, what is the protocol used to produce the result images on the product page?
B. Brown
Verified customer
Asked: 2015-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-09-11
Question
Will anti-Lamin A+C/LMNA antibody PB9280 work for WB with mucosa of stomach?
Z. Anderson
Verified customer
Asked: 2014-08-11
Answer
According to the expression profile of mucosa of stomach, LMNA is highly expressed in mucosa of stomach. So, it is likely that anti-Lamin A+C/LMNA antibody PB9280 will work for WB with mucosa of stomach.
Boster Scientific Support
Answered: 2014-08-11
Question
I was wanting to use your anti-Lamin A+C/LMNA antibody for WB for mouse mucosa of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse mucosa of stomach identification?
J. Krishna
Verified customer
Asked: 2014-04-18
Answer
As indicated on the product datasheet, PB9280 anti-Lamin A+C/LMNA antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse mucosa of stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-18
