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Product Info Summary
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Anti-PPAR gamma/PPARG Antibody
Boster Bio Anti-PPAR gamma/PPARG Antibody catalog # PA1320. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPAR gamma/PPARG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1320)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma (45-62aa PHYEDIPFTRTDPVVADY), different from the rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
PA1320 is reactive to PPARG in Human
PA1320 is guaranteed for WB Boster Guarantee
Background of PPAR gamma/NR1C3
The peroxisome proliferator-activated receptors (PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al. (2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-PPAR gamma antibody, PA1320, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
Gene/Protein Information For PPARG (Source: Uniprot.org, NCBI)
Peroxisome proliferator-activated receptor gamma
nuclear hormone receptor family
CIMT1; NR1C3; NR1C3GLM1; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma; PPAR gamma; PPARG; PPARG1peroxisome proliferative activated receptor, gamma; PPARG2PPARgamma; PPAR-gamma PPARG CIMT1, GLM1, NR1C31, PPARG2, PPARG5, PPARgamma, PPARG peroxisome proliferator activated receptor gamma peroxisome proliferator-activated receptor gamma|PPAR-gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferator-activated nuclear receptor gamma variant 1|peroxisome proliferator-activated receptor-gamma 5|peroxisome proliferator-activated receptor-gamma splicing*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on PPARG, check out the PPARG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PA1320 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
PA1320 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
Rosiglitazone infusion therapy following minimally invasive surgery for intracerebral hemorrhage evacuation decreases matrix metalloproteinase-9 and blood-brain barrier disruption in rabbits.
Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice
Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells
Scorpion in Combination with Gypsum: Novel Antidiabetic Activities in Streptozotocin-Induced Diabetic Mice by Up-Regulating Pancreatic PPAR
Wang W, Liu L, Dang X, Ma S, Zhang M, Wang K. Bmc Musculoskelet Disord. 2012 Aug 9;13:142. Doi: 10.1186/1471-2474-13-142. The Effect Of Core Decompression On Local Expression Of Bmp-2, Ppar-?? And Bone Regeneration In The Steroid-Induced Femoral H...
Jiang Y, Zhang Y, Zhang H, Zhu B, Li P, Lu C, Xu Y, Chen W, Lin N. Exp Biol Med (Maywood). 2014 Mar;239(3):347-55. Doi: 10.1177/1535370213519215. Epub 2014 Feb 7. Pravastatin Prevents Steroid-Induced Osteonecrosis In Rats By Suppressing Ppar?? Exp...
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