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LMNB1 antibody (PA1048) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 45 Publications

pa1048 lmnb1 primary antibodies if testing 1_1

IF analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048).
Lamin B1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Lamin B1/LMNB1 Antibody Picoband®

LMNB1 antibody

45 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IF, IHC, ICC, WB

Tested Applications

LMNB1 antibody was positively detected by WB in:

human Hela whole cell, human K562 whole cell, human SH-SY5Y whole cell, rat spleen tissue, rat PC-12 whole cell, mouse RAW264.7 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

LMNB1 antibody was positively detected by IHC in:

human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

LMNB1 antibody was positively detected by IF in:

human lung cancer tissue, rat brain tissue, mouse brain tissue

LMNB1 antibody was positively detected by ICC/IF in:

U2OS cell

LMNB1 antibody was positively detected by IP in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Mouse

Tested

Rabbit

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Lamin B1/LMNB1 Antibody Picoband®
View all LMNB1 Antibodies

Form

Lyophilized

Uniprot

P20700

Gene ID

4001

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1, different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

70-72 kDa

Calculated Molecular Weight

66.4 kDa

Background of LMNB1

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Cite This Product

Anti-Lamin B1/LMNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1048)

Protocols

Western Blot protocol for LMNB1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for LMNB1 antibody

Review Protocol

Immunofluorescence (IF) protocol for LMNB1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for LMNB1 antibody

Review Protocol

Immunoprecipitation (IP) protocol for LMNB1 antibody

Review Protocol

Publications

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Published: 2026-03-16

Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY

Application: WB

Impact Factor: 3.10

Species: Mouse

PMID: 41838132

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Anti-Lamin B1/LMNB1 Antibody Picoband®

LMNB1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Cell-free supernatant of Clostridium butyricum induces mitochondrial apoptosis and suppresses NF-κB pathway in colorectal cancer cells

Cellular hnRNP AB inhibits avian influenza virus RNA synthesis via blocking UAP56-mediated nuclear export of PB2 mRNA

Qifenggubiao granules attenuate LPS-induced inflammation through α7nAChR activation: An integrated study of network pharmacology and phytopharmacological validation

ID1 boosts antiviral immunity by countering PRMT5-mediated STING methylation

hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis

Berberine triggers apoptosis through the PI3K/Akt pathways and Nrf2 by inducing ROS in papillary thyroid cancer

Sirtuin-1 Regulates Mitochondrial Calcium Uptake Through Mitochondrial Calcium Uptake 1 (MICU1)

Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma

The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling

SIRT7: A potential prognostic marker and therapeutic target in gallbladder cancer

A novel GATA3 frameshift mutation causes hypoparathyroidism, sensorineural deafness, and renal dysplasia syndrome

The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

Dexmedetomidine abates myocardial ischemia reperfusion injury through inhibition of pyroptosis via regulation of miR-665/MEF2D/Nrf2 axis

Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling

Dexmedetomidine attenuates myocardial ischemia/reperfusion-induced ferroptosis via AMPK/GSK-3β/Nrf2 axis

SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism

Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways

MicroRNA-1246 suppresses the metastasis of breast cancer cells by targeting the DYRK1A/PGRN axis to prevent the epithelial-mesenchymal transition

Cellular hnRNPAB interacts with avian influenza viral protein PB2 and inhibits virus replication potentially by restricting PB2 mRNA nuclear export and PB2 protein level

Pyrotinib enhances the radiosensitivity of HER2‑overexpressing gastric and breast cancer cells

Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway

Enhancement of anti-acne effect of Scutellaria baicalensis extract by fermentation with symbiotic fungus Penicillium decumbens

Neddylation promotes protein translocation between the cytoplasm and nucleus

PBX1 promotes development of natural killer cells by binding directly to the Nfil3 promoter

Chronic periodontitis induces microbiota-gut-brain axis disorders and cognitive impairment in mice

Diabetes aggravates renal ischemia and reperfusion injury in rats by exacerbating oxidative stress, inflammation, and apoptosis

Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway

Inhibition of HDAC6 attenuates LPS-induced inflammation in macrophages by regulating oxidative stress and suppressing the TLR4-MAPK/NF-κB pathways

Propionibacterium acnes related anti-inflammation and skin hydration activities of madecassoside, a pentacyclic triterpene saponin from Centella asiatica

Targeting KPNB1 overcomes TRAIL resistance by regulating DR5, Mcl-1 and FLIP in glioblastoma cells

3-Dehydroandrographolide protects against lipopolysaccharide-induced inflammation through the cholinergic anti-inflammatory pathway

Procyanidin B2 mitigates behavioral impairment and protects myelin integrity in cuprizone-induced schizophrenia in mice

Effect of polysaccharides from Vitis vinifera L. on NF-κB/IκB-α signal pathway and inflammatory factors in Alzheimer's model rats

Curcumin enhances the anti-cancer effects of Paris Saponin II in lung cancer cells

Renal ischemia-reperfusion injury attenuated by splenic ischemic preconditioning

Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction

Regulation of ABCG2 by nuclear factor kappa B affects the sensitivity of human lung adenocarcinoma A549 cells to arsenic trioxide

Glutamine ameliorates lipopolysaccharide‑induced cardiac dysfunction by regulating the toll‑like receptor 4/mitogen‑activated protein kinase/nuclear factor‑κB signaling pathway

Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose

Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart

Cytoplasmic translocation of HuR contributes to angiotensin II induced cardiac fibrosis

Zearalenone induces oxidative damage involving Keap1/Nrf2/HO-1 pathway in hepatic L02 cells

Tert-butylhydroquinone attenuates scrotal heat-induced damage by regulating Nrf2-antioxidant system in the mouse testis

Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility

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