Anti-Lamin B1/LMNB1 Antibody

Boster Bio Anti-Lamin B1/LMNB1 Antibody catalog # PA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 24 publication(s).

Product Info Summary

SKU: PA1048
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Lamin B1/LMNB1 Antibody

See all Lamin B1 products

SKU/Catalog Number

PA1048

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lamin B1/LMNB1 Antibody catalog # PA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lamin B1/LMNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1048)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1 (570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1048 is reactive to LMNB1 in Human, Mouse, Rat

Applications

PA1048 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For LMNB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20700

Gene ID

4001

Gene Name

LMNB1

Full Name

Lamin-B1

Weight

66.408kDa

Superfamily

intermediate filament family

Alternative Names

ADLD; Lamin B1; lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419 LMNB1 ADLD, LMN, LMN2, LMNB, MCPH26 lamin B1 lamin-B1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LMNB1, check out the LMNB1 Infographic

LMNB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LMNB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1048 has been cited in 24 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang T,Luo X,Wu B,Peng P,Dai Y,Hu G,Qiu H,Yuan X.Pyrotinib enhances the radiosensitivity of HER2‑overexpressing gastric and breast cancer cells.Oncol Rep.2020 Dec;44(6):2634-2644.doi:10.3892/or.2020.7820.Epub 2020 Oct 21.PMID:33125154;PMCID:PMC7640366.
Species: Human,Mouse

Na,L.,Q,B.,Xiumei,Z.et al.Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period.BMC Cardiovasc Disord 20, 139 (2020).https://doi.org/10.1186/s12872-020-01418-z
Species: Rat
PA1048 usage in article: APP:WB, SAMPLE:HEART TISSUE, DILUTION:NA

Zhang B,Zhong Q,Chen X,Wu X,Sha R,Song G,Zhang C,Chen X.Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway.Front Neurosci.2020 Aug 11;14:847.doi:10.3389/fnins.2020.00847.PMID:328485
Species: Rat
PA1048 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:500

Zhong J,Ye S,Zhou X,Huang J,Li X,Zhang S,Zhou J,Chen D,Li C.Plastrum Testudinis Extracts Promote NSC Differentiation into Dopaminergic Neuron by Regulating the Interaction of TET1 and FoxA2.Evid Based Complement Alternat Med.2020 Apr 20;2020:9104135.doi:1
Species: Rat
PA1048 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Xu X,Zhou Y,Fu B,Zhang J,Dong Z,Zhang X,Shen N,Sun R,Tian Z,Wei H. PBX1 promotes development of natural killer cells by binding directly to the Nfil3 promoter. FASEB J.2020 May;34(5):6479-6492. doi: 10.1096/fj. 202000121R.Epub 2020 Mar 19. PMID:32190943.
Species: Mouse
PA1048 usage in article: APP:WB, SAMPLE:293T CELL, DILUTION:NA

Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
PA1048 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:250

Glutamine ameliorates lipopolysaccharide-induced cardiac dysfunction by regulating the toll-like receptor 4/mitogen-activated protein kinase/nuclear factor-kB signaling pathway

Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway

Overexpression of DJ-1 reduces oxidativestressand attenuates hypoxia/reoxygenation injury in NRK-52Ecellsexposed to high glucose

Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart

Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer.

The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration

Simulated microgravity inhibits osteogenic differentiation of mesenchymal stem?cells?via depolymerizing F-actin to impede TAZ nuclear translocation

Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus)

Trimetazidine prevents macrophage?mediated septic myocardial dysfunction via activation of the histone deacetylase sirtuin 1

Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells

Wang Y, Wu T, Hu D, Weng X, Wang X, Chen PJ, Luo X, Wang H, Ning Q. J Lipid Res. 2018 Jan;59(1):58-68. doi: 10.1194/jlr.M079533. Epub 2017 Nov 13. Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver vi...

Bai Y, Du S, Li F, Huang F, Deng R, Zhou J, Chen D. Exp Biol Med (Maywood). 2017 Mar;242(5):527-535. doi: 10.1177/1535370216685433. Epub 2017 Jan 5. Histone deacetylase-high mobility group box-1 pathway targeted by hypaconitine suppresses the apop...

Zhou HJ, Li H, Shi MQ, Mao XN, Liu DL, Chang YR, Gan YM, Kuang X, Du JR. Front Pharmacol. 2018 Jan 18;8:950. doi: 10.3389/fphar.2017.00950. eCollection 2017. Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition o...

Gao S, Wu J, Liu Ry, Li J, Song L, Teng Y, Sheng C, Liu D, Yao C, Chen H, Jiang W, Chen S, Huang W. J Virol. 2015 Jan;89(1):300-11. Doi: 10.1128/Jvi.02170-14. Epub 2014 Oct 15. Interaction Of Ns2 With Aimp2 Facilitates The Switch From Ubiquitinati...

Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...

Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...

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5 Customer Q&As for Anti-Lamin B1/LMNB1 Antibody

Question

Can you help my question with product PA1048, anti-Lamin B1/LMNB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1048 anti-Lamin B1/LMNB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-22

Question

Will PA1048 anti-Lamin B1/LMNB1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

As indicated on the product datasheet, PA1048 anti-Lamin B1/LMNB1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-09

Question

Is a blocking peptide available for product anti-Lamin B1/LMNB1 antibody (PA1048)?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

We do provide the blocking peptide for product anti-Lamin B1/LMNB1 antibody (PA1048). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Lamin B1/LMNB1 antibody PA1048. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-15

Question

I was wanting to use your anti-Lamin B1/LMNB1 antibody for IHC for rat colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat colon carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

As indicated on the product datasheet, PA1048 anti-Lamin B1/LMNB1 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-28

Size

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