radio
SKU PA1048
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, ICC, WB

Overview

Product Name Anti-Lamin B1/LMNB1 Antibody
SKU/Catalog Number PA1048
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-Lamin B1/LMNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1048)
Host Rabbit
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

/antibody/pa1048 1 WB anti lamin b1 antibody.jpg

Anti-Lamin B1 antibody, PA1048, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate

/antibody/pa1048 2 IHC anti lamin b1 antibody.jpg

Anti-Lamin B1 antibody, PA1048, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/antibody/pa1048 3 IHC anti lamin b1 antibody.jpg

Anti-Lamin B1 antibody, PA1048, IHC(F)
IHC(F): Rat Intestine Tissue

/antibody/pa1048 4 IF anti lamin b1 antibody.jpg

Anti-Lamin B1 antibody, PA1048, ICC
ICC: A549 Cell

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P20700
Gene Name LMNB1
Protein Name Lamin-B1
Alternative Names Lamin-B1;LMNB1;LMN2, LMNB;
Subcellular Localization Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Molecular Weight 66408 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Background Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Order Product (PA1048)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Glutamine ameliorates lipopolysaccharide-induced cardiac dysfunction by regulating the toll-like receptor 4/mitogen-activated protein kinase/nuclear factor-kB signaling pathway
Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway
The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration
Simulated microgravity inhibits osteogenic differentiation of mesenchymal stemcellsvia depolymerizing F-actin to impede TAZ nuclear translocation
Simulated microgravity inhibits osteogenic differentiation of mesenchymal stem cells via depolymerizing F-actin to impede TAZ nuclear translocation
Overexpression of DJ-1 reduces oxidativestressand attenuates hypoxia/reoxygenation injury in NRK-52Ecellsexposed to high glucose
Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus)
Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart
Trimetazidine prevents macrophage?mediated septic myocardial dysfunction via activation of the histone deacetylase sirtuin 1
Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer.
Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt
Interaction of NS2 with AIMP2 Facilitates the Switch from Ubiquitination to SUMOylation of M1 in Influenza A Virus-Infected Cells
Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells
Wang Y, Wu T, Hu D, Weng X, Wang X, Chen PJ, Luo X, Wang H, Ning Q. J Lipid Res. 2018 Jan;59(1):58-68. doi: 10.1194/jlr.M079533. Epub 2017 Nov 13. Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein[S]
Bai Y, Du S, Li F, Huang F, Deng R, Zhou J, Chen D. Exp Biol Med (Maywood). 2017 Mar;242(5):527-535. doi: 10.1177/1535370216685433. Epub 2017 Jan 5. Histone deacetylase-high mobility group box-1 pathway targeted by hypaconitine suppresses the apoptosis of endothelial cells
Zhou HJ, Li H, Shi MQ, Mao XN, Liu DL, Chang YR, Gan YM, Kuang X, Du JR. Front Pharmacol. 2018 Jan 18;8:950. doi: 10.3389/fphar.2017.00950. eCollection 2017. Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition of RIG-I/NF-κB Signaling.
Ye Bai, Shaohui Du, Fei Li, Fengyuan Huang, Rudong Deng, Jianhong Zhou, Dongfeng Chen Histone deacetylase-high mobility group box-1 pathway targeted by hypaconitine suppresses the apoptosis of endothelial cells Experimental Biology and Medicine
Gao S, Wu J, Liu Ry, Li J, Song L, Teng Y, Sheng C, Liu D, Yao C, Chen H, Jiang W, Chen S, Huang W. J Virol. 2015 Jan;89(1):300-11. Doi: 10.1128/Jvi.02170-14. Epub 2014 Oct 15. Interaction Of Ns2 With Aimp2 Facilitates The Switch From Ubiquitinati...
Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lamin b1 antibody, lamin-b1 antibody, lmnb1 antibody