|Product Name||Anti-Lamin B1/LMNB1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-Lamin B1/LMNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1048)|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunocytochemistry , 0.5-1μg/ml, Human, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Images And Assay Conditions
Anti-Lamin B1 antibody, PA1048, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-Lamin B1 antibody, PA1048, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Lamin B1 antibody, PA1048, IHC(F)
IHC(F): Rat Intestine Tissue
Anti-Lamin B1 antibody, PA1048, ICC
ICC: A549 Cell
Protein Target Info (Source: Uniprot.org)
|Alternative Names||Lamin-B1;LMNB1;LMN2, LMNB;|
|Subcellular Localization||Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.|
|Molecular Weight||66408 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.|
|Background||Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,