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Lamc1 antibody (A03522) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 34 Publications

a03522 laminin primary antibodies if testing 6

IF analysis of Laminin using anti-Laminin antibody (A03522).
Laminin was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Laminin Antibody (A03522) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®

Lamc1 antibody

34 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

Lamc1 antibody was positively detected by WB in:

human A431 whole cell, human A549 whole cell, human PC-3 whole cell, human CACO-2 whole cell, rat NRK whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse HEPA1-6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Lamc1 antibody was positively detected by IHC in:

mouse heart tissue, mouse kidney tissue, rat cardiac muscle tissue, rat kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Lamc1 antibody was positively detected by IF in:

mouse skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Mouse

Tested

Product Information

Product Name

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
View all LAMC1 Antibodies

Form

Lyophilized

Uniprot

P02468

Gene ID

226519

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Peptide mixture of laminin gamma1,2,3 (NKLNEIEGSLNKAKDEMKAS; DLEERVRRQRNHLHLLETSI; LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

150, 220-250 kDa

Calculated Molecular Weight

177.3 kDa

Background of Lamc1

Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Cite This Product

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03522)

Protocols

Western Blot protocol for Lamc1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Lamc1 antibody

Review Protocol

Immunofluorescence (IF) protocol for Lamc1 antibody

Review Protocol

Publications

Lactate-activated GPR81/FARP1 signaling drives insulin-independent glucose uptake and metabolic control

Published: 2026-01-13

Journal: CELL RESEARCH

Application: IF

Impact Factor: 25.90

Species: Mouse

PMID: 41530347

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Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®

Lamc1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Lactate-activated GPR81/FARP1 signaling drives insulin-independent glucose uptake and metabolic control

Cinobufagin modulates vasculogenic mimicry and tumor-associated macrophages to inhibit ovarian cancer progression

Mitochondrial ROS Accumulation Contributes to Maternal Hypertension and Impaired Remodeling of Spiral Artery but Not IUGR in a Rat PE Model Caused by Maternal Glucocorticoid Exposure

Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork

Effects of Ocular Hypertension on Cytoskeleton and Stiffness of Trabecular Meshwork Cells in Rats

Alendronate crosslinked chitosan/polycaprolactone scaffold for bone defects repairing

miR-194-Loaded Gelatin Nanospheres Target MEF2C to Suppress Muscle Atrophy in a Mechanical Unloading Model

BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue

The notch pathway promotes NF-κB activation through Asb2 in T cell acute lymphoblastic leukemia cells

Nanoplatform Assembled from a CD44-Targeted Prodrug and Smart Liposomes for Dual Targeting of Tumor Microenvironment and Cancer Cells

Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord

The role of ursodeoxycholic acid on cholestatic hepatic fibrosis in infant rats

Oxidized low-density lipoprotein suppresses mouse granulosa cell differentiation through disruption of the hypoxia-inducible factor 1 pathway

Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells

Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury

Apoptosis and death receptor signaling in diaphragm of burnt rats

Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury

Enhancement of endothelial differentiation of adipose derived mesenchymal stem cells by a three-dimensional culture system of microwell

Observation of vascularization and protein after implanting porous silk fibroin films in&nbsp;rat

Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation

Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells

Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses

Electrosprayed Microparticles with Loaded pDNA-Calcium Phosphate Nanoparticles to Promote the Regeneration of Mature Blood Vessels

Electroacupuncture promotes the differentiation of transplanted bone marrow mesenchymal stem cells overexpressing TrkC into neuron-like cells in transected spinal cord of rats.

Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection

Bone Marrow Mesenchymal Stem Cells and Electroacupuncture Downregulate the Inhibitor Molecules and Promote the Axonal Regeneration in the Transected Spinal Cord of Rats

Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord

Effects of in vitro cultivated Calculus Bovis compound on pulmonary lesions in rabbits with schistosomiasis

Microplasmin Degrades Fibronectin and Laminin at Vitreoretinal Interface and Outer Retina during Enzymatic Vitrectomy

Effect of Arachidonic Acid on Production of Laminin and Connexin of Granulosa Cells from Chicken Pre-hierarchical Follicles

Effect of burn injury on apoptosis and expression of apoptosis-related genes/proteins in skeletal muscles of rats

Effect of Chinese traditional compound, Gan-fu-kang, on CCl4-induced liver fibrosis in rats and its probable molecular mechanisms

Targeted Disruption of Smad4 in Cardiomyocytes Results in Cardiac Hypertrophy and Heart Failure

Early protective effect of ischemic preconditioning on small intestinal graft in rats

Review

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Observation of vascularization and protein after implanting porous silk fibroin films in rat