Product Info Summary
SKU: | FEK1118 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
Assay Time: | 90 Minutes |
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Product info
Product Name
Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit
See all alpha 2-Macroglobulin products
SKU/Catalog Number
FEK1118
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human A2M/Alpha2-Macroglobulin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1118)
Clonality of Antibodies
See Datasheet for details
Immunogen
Target protein isolated from human plasma
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1118 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1118 is reactive to A2M in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1118 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of alpha 2-Macroglobulin
Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Kit Components
Catalog number | Description | Quantity | Volume |
---|---|---|---|
FEK1118-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
EK1118-ST | Human alpha 2-Macroglobulin Standard | 2 | 40 ng/tube |
FEK1118-A | Human A2M Antibody A | 1 | 3ml |
FEK1118-B | Human A2M Antibody B | 1 | 3ml |
AR1106-1 | Sample Diluent | 1 | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
AR1105 | Stop Solution | 1 | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.067 | 0.190 | 0.267 | 0.419 | 0.709 | 1.285 | 2.017 | 2.532 |
Data Example Images
Click image to see more details
Human A2M/alpha2-Macroglobulin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1336 | 3774 | 26412 | 1412 | 3693 | 26457 |
Standard deviation | 93.52 | 233.99 | 1980.9 | 108.72 | 249.67 | 2090.1 |
CV (%) | 7% | 6.2% | 7.5% | 7.7% | 6.3% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of alpha 2-Macroglobulin in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1336 | 1254 | 1452 | 1396 | 1360 | 61.18 | 4.5% |
Sample 2 | 3774 | 3674 | 3364 | 3354 | 3542 | 254.99 | 7.2% |
Sample 3 | 26412 | 33012 | 29451 | 29456 | 29583 | 1804.55 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For A2M (Source: Uniprot.Org, NCBI)
Gene Name
A2M
Full Name
Alpha-2-macroglobulin
Weight
163.291kDa
Superfamily
protease inhibitor I39 (alpha-2-macroglobulin) family
Alternative Names
A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7 A2M A2MD, CPAMD5, FWP007, S863-7 alpha-2-macroglobulin alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|alpha-2-M
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on A2M, check out the A2M Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for A2M: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (FEK1118)
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