Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit

alpha 2-Macroglobulin ELISA kit, 90-minute one-step protocol

Human A2M/Alpha2-Macroglobulin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: FEK1118
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

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Product Name

Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit

See all alpha 2-Macroglobulin products

SKU/Catalog Number

FEK1118

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human A2M/Alpha2-Macroglobulin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1118)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: from human plasma

Sensitivity

<20 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1118 is reactive to A2M in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK1118 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of alpha 2-Macroglobulin

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Kit Components

Catalog numberDescription QuantityVolume
FEK1118-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1118-STHuman alpha 2-Macroglobulin Standard 2 40 ng/tube
FEK1118-CHuman A2M Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0670.1900.2670.4190.7091.2852.0172.532

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13363774264121412369326457
Standard deviation93.52233.991980.9108.72249.672090.1
CV (%)7%6.2%7.5%7.7%6.3%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of alpha 2-Macroglobulin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11336125414521396136061.184.5%
Sample 237743674336433543542254.997.2%
Sample 326412330122945129456295831804.556.1%
*number of samples for each test n=16.

Gene/Protein Information For A2M (Source: Uniprot.Org, NCBI)

Uniprot ID

P01023

Gene ID

2

Gene Name

A2M

Full Name

Alpha-2-macroglobulin

Weight

163.291kDa

Superfamily

protease inhibitor I39 (alpha-2-macroglobulin) family

Alternative Names

A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7 A2M A2MD, CPAMD5, FWP007, S863-7 alpha-2-macroglobulin alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|alpha-2-M

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on A2M, check out the A2M Infographic

A2M infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for A2M: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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