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Pack Size:100μg/vial
Validated Species:Human, Mouse, Rat
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Product Name Anti-T-bet/Tbx21 Antibody
SKU/Catalog Number PA1999
Description Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-T-bet/Tbx21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1999)
Replacement Item This antibody may replace the following items: sc-7960|sc-6201|sc-7960-X|sc-6031-R|sc-6031-X|sc-6201-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet(522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TBX21
Protein Name T-box transcription factor TBX21
Molecular Weight 58328 MW
Protein Function Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Tissue Specificity T-cell specific.
Subcellular Localization Nucleus.
Uniprot ID Q9UL17
Alternative Names T-box transcription factor TBX21;T-box protein 21;T-cell-specific T-box transcription factor T-bet;Transcription factor TBLYM;TBX21;TBET, TBLYM;
Research Areas |immunology|adaptive immunity|b cells|non-cd| immunology|t cells| epigenetics and nuclear signaling|transcription|domain families|developmental families| stem cells|hematopoietic progenitors|surface molecules|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for T-box transcription factor TBX21

TBX21(T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang(2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al.(2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer(NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al.(2002) observed reduced expression of the T(H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Anti-T-bet/Tbx21 Antibody Images

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Anti-T-bet/Tbx21 Antibody
Anti-T-bet/Tbx21 antibody, PA1999, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TBX21,34.7KD (162aa tag+ V381-N535)
Lane 1: Recombinant Human TBX21 Protein 5ng
Lane 2: Recombinant Human TBX21 Protein 2.5ng
Lane 3: Recombinant Human TBX21 Protein 1.25ng
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Miao L, Run-Ming J, Yi J. Iran J Pediatr. 2012 Mar;22(1):43-51. T-Bet Mediated Anti-Neoplastic Effects Of Dendritic Cell-Cytokine Induced Killer Cells In Vitro.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to t bet antibody, tbet antibody