|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-LCAT Antibody|
|Description||Rabbit IgG polyclonal antibody for Phosphatidylcholine-sterol acyltransferase(LCAT) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-LCAT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1967)|
|Replacement Item||This antibody may replace the following items: sc-98873|sc-47900|sc-98874|sc-377509|sc-376682|sc-47899|sc-398361 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT(300-313aa RDFQRFFADLHFEE), different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Phosphatidylcholine-sterol acyltransferase|
|Molecular Weight||49578 MW|
|Protein Function||Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. .|
|Tissue Specificity||Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. Expressed in Hep- G2 cell line. .|
|Sequence Similarities||Belongs to the AB hydrolase superfamily. Lipase family.|
|Subcellular Localization||Secreted . Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid (CSF).|
|Alternative Names||Phosphatidylcholine-sterol acyltransferase;188.8.131.52;Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase;LCAT;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| signal transduction| cardiovascular|atherosclerosis|lipoprotein metabolism| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|heart disease||
Background for Phosphatidylcholine-sterol acyltransferase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LCAT Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LCAT Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,