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Product Info Summary
| SKU: | M00516-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA (Cap) |
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Product info
Product Name
Anti-PON1 Antibody (monoclonal, 11H2)
SKU/Catalog Number
M00516-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PON1 Antibody (monoclonal, 11H2) catalog # M00516-2. Tested in ELISA applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PON1 Antibody (monoclonal, 11H2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00516-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
11H2
Isotype
Mouse IgG2a
Immunogen
E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00516-2 is reactive to Pon1 in Mouse, Rat
Calculated molecular weight
39.6 kDa
Background of Pon1
Isocitrate dehydrogenase [NADP], mitochondrialis anenzymethat in humans is encoded by theIDH2gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00516-2 is guaranteed for ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Sandwich ELISA - Recombinant mouse PON1 protein standard curve.
Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1621).
Specific Publications For Anti-PON1 Antibody (monoclonal, 11H2) (M00516-2)
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4 Customer Q&As for Anti-PON1 Antibody (monoclonal, 11H2)
Question
Will anti-PON1 antibody (monoclonal, 11H2) M00516-2 work for ELISA with brain?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
According to the expression profile of brain, PON1 is highly expressed in brain. So, it is likely that anti-PON1 antibody (monoclonal, 11H2) M00516-2 will work for ELISA with brain.
Boster Scientific Support
Answered: 2019-12-27
Question
Would M00516-2 anti-PON1 antibody (monoclonal, 11H2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
As indicated on the product datasheet, M00516-2 anti-PON1 antibody (monoclonal, 11H2) as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-02
Question
I was wanting to use your anti-PON1 antibody (monoclonal, 11H2) for ELISA for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can see on the product datasheet, M00516-2 anti-PON1 antibody (monoclonal, 11H2) has been tested for ELISA on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Is there a BSA free version of anti-PON1 antibody (monoclonal, 11H2) M00516-2 available?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PON1 antibody (monoclonal, 11H2) M00516-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-11
