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Product Info Summary
| SKU: | A02143 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Gastric Mucin/MUC6 Antibody Picoband®
SKU/Catalog Number
A02143
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Gastric Mucin/MUC6 Antibody Picoband® catalog # A02143. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gastric Mucin/MUC6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02143)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Gastric Mucin recombinant protein (Position: H52-R230).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02143 is reactive to MUC6 in Human, Mouse
Observed Molecular Weight
257 kDa
Calculated molecular weight
257.1 kDa
Background of MUC6
Mucin 6, oligomeric mucus/gel-forming, also known as MUC6, is a human gene. This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02143 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse stomach tissue, human SGC-7901 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse stomach tissue lysates,
Lane 2: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.
Click image to see more details
Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Normal group-rat colon tissue lysates,
Lane 2: Model group-rat colon tissue lysates,
Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates,
Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates,
Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates,
Lane 6: Western medicine treatment-rat colon tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 1:1000 overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.
Specific Publications For Anti-Gastric Mucin/MUC6 Antibody Picoband® (A02143)
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Customer Reviews
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1 Reviews For Anti-Gastric Mucin/MUC6 Antibody Picoband®
This antibody is highly efficient and specific, suitable for Western blot detection of MUC6 protein in rat colon tissue, with only minor nonspecific bands.
Excellent
| SKU | A02143 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Gastric Mucin/MUC6 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows the Western blot results of the target protein MUC6 and the internal control Actin in rat colon tissue across different groups. The target bands are clear and well defined, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-06
Customer Q&As
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1 Customer Q&As for Anti-Gastric Mucin/MUC6 Antibody Picoband®
Question
We are currently using anti-Gastric Mucin/MUC6 antibody A02143 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
C. Lewis
Verified customer
Asked: 2015-12-17
Answer
The anti-Gastric Mucin/MUC6 antibody (A02143) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-17
