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Product Info Summary
| SKU: | PB9036 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-LIF Antibody Picoband®
SKU/Catalog Number
PB9036
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LIF Antibody Picoband® catalog # PB9036. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9036)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9036 is reactive to LIF in Human
Observed Molecular Weight
36 kDa
Calculated molecular weight
22.0 kDa
Background of LIF
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9036 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (Paraffin-embedded Section) | 0.5-1μg/ml | Human |
| Western blot | 0.1-0.5μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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IHC analysis of LIF using anti-LIF antibody (PB9036).
LIF was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIF Antibody (PB9036) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of LIF using anti-LIF antibody (PB9036).
LIF was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIF Antibody (PB9036) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of LIF using anti-LIF antibody (PB9036).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody (Catalog # PB9036) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIF at approximately 36 kDa. The expected band size for LIF is at 22 kDa.
Specific Publications For Anti-LIF Antibody Picoband® (PB9036)
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5 Customer Q&As for Anti-LIF Antibody Picoband®
Question
We have tried in the past anti-LIF antibody for WB on left coronary artery in the past. I am using human, and We are going to use the antibody for IHC-P next. I would like examining left coronary artery as well as embryonic stem cell in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
I took a look at the website and datasheets of our anti-LIF antibody and I see that PB9036 has been validated on human in both WB and IHC-P. Thus PB9036 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-10
Question
We are currently using anti-LIF antibody PB9036 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-LIF antibody (PB9036) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
My question regards using your anti-LIF antibody for regulation of metanephric nephron tubule epithelial cell differentiation studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Thank you for your inquiry. This PB9036 anti-LIF antibody is tested on human placenta tissue, hela whole cell lysates, a549 whole cell lysates. It is guaranteed to work for IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-09
Question
We have seen staining in human colon. Are there any suggestions? Is anti-LIF antibody supposed to stain colon positively?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Based on literature colon does express LIF. Based on Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-08-21
Question
My team were happy with the WB result of your anti-LIF antibody. However we have observed positive staining in colon secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to literature, colon does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-08-09
