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Product Info Summary
| SKU: | PA1562 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Leukemia inhibitory factor LIF Antibody Picoband®
SKU/Catalog Number
PA1562
BA1239-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leukemia inhibitory factor LIF Antibody catalog # PA1562. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leukemia inhibitory factor LIF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1562)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF, identical to the related rat sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1562 is reactive to Lif in Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
22.3 kDa
Background of Lif
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1562 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Intestine Tissue, Rat Brain Tissue, Rat Spleen Tissue, Rat Thymus Tissue, Mouse Brain Tissue, Mouse Kidney Tissue, Mouse Liver Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-LIF antibody, PA1562, Western blotting
All lanes: Anti LIF (PA1562) at 0.5ug/ml
Lane 1: Rat Intestine Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Thymus Tissue Lysate at 50ug
Lane 5: Mouse Brain Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 40KD
Specific Publications For Anti-Leukemia inhibitory factor LIF Antibody Picoband® (PA1562)
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4 Customer Q&As for Anti-Leukemia inhibitory factor LIF Antibody Picoband®
Question
My boss were well pleased with the WB result of your anti-LIF antibody. However we have seen positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?
K. Miller
Verified customer
Asked: 2019-10-31
Answer
From literature, left coronary artery does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-10-31
Question
We have seen staining in rat embryonic stem cell. What should we do? Is anti-LIF antibody supposed to stain embryonic stem cell positively?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From what I have seen in literature embryonic stem cell does express LIF. From what I have seen in Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-LIF antibody PA1562 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
The anti-LIF antibody (PA1562) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-25
Question
I am looking for using your anti-LIF antibody for positive regulation of astrocyte differentiation studies. Has this antibody been tested with western blotting on intestine tissue? We would like to see some validation images before ordering.
Z. Kulkarni
Verified customer
Asked: 2013-10-16
Answer
We appreciate your inquiry. This PA1562 anti-LIF antibody is tested on rat thymus tissue, intestine tissue, tissue lysate, brain tissue, spleen tissue, mouse liver tissue, kidney tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-10-16
