Anti-STAT1 Antibody Picoband™

Boster Bio Anti-STAT1 Antibody Picoband™ catalog # A00036-2. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: A00036-2
Size: 100μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-STAT1 Antibody Picoband™

See all STAT1 products

SKU/Catalog Number

A00036-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAT1 Antibody Picoband™ catalog # A00036-2. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00036-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00036-2 is reactive to STAT1 in Human, Monkey, Mouse, Rat

Applications

A00036-2 is guaranteed for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For STAT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P42224

Gene ID

6772

Gene Name

STAT1

Full Name

Signal transducer and activator of transcription 1-alpha/beta

Weight

87.335kDa

Superfamily

transcription factor STAT family

Alternative Names

CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STAT1, check out the STAT1 Infographic

STAT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for STAT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00036-2 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway

Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

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Find answers in Q&As, reviews.

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16 Customer Q&As for Anti-STAT1 Antibody Picoband™

Question

Do you have a BSA free version of anti-STAT1 antibody A00036-2 available?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-STAT1 antibody A00036-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-13

Question

My question regards to test anti-STAT1 antibody A00036-2 on monkey placenta testis for research purposes, then I may be interested in using anti-STAT1 antibody A00036-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

The products we sell, including anti-STAT1 antibody A00036-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-06

Question

Our lab were content with the WB result of your anti-STAT1 antibody. However we have been able to see positive staining in cervix carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

From what I have seen in literature, cervix carcinoma does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-21

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-STAT1 antibody A00036-2. Let me know if you need anything else.

L. Krishna

Verified customer

Asked: 2019-11-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-06

Question

We are currently using anti-STAT1 antibody A00036-2 for monkey tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The anti-STAT1 antibody (A00036-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-17

Question

Would A00036-2 anti-STAT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

As indicated on the product datasheet, A00036-2 anti-STAT1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-30

Question

Can you help my question with product A00036-2, anti-STAT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Johnson

Verified customer

Asked: 2019-04-09

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00036-2 anti-STAT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-09

Question

I see that the anti-STAT1 antibody A00036-2 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-25

Question

Our lab want to know about using your anti-STAT1 antibody for response to mechanical stimulus studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

K. Parker

Verified customer

Asked: 2019-02-18

Answer

Thanks for your inquiry. This A00036-2 anti-STAT1 antibody is validated on human hela, hela whole cell lysates, a549 whole cell lysates, k562 whole cell lysates, a431 cells, rat thymus tissue, mouse thymus tissue. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-02-18

Question

Is a blocking peptide available for product anti-STAT1 antibody (A00036-2)?

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

We do provide the blocking peptide for product anti-STAT1 antibody (A00036-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-07

Question

I was wanting to use your anti-STAT1 antibody for ICC for monkey placenta testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for monkey placenta testis identification?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

As indicated on the product datasheet, A00036-2 anti-STAT1 antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in monkey placenta testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-06

Question

Here is the WB image, lot number and protocol we used for placenta testis using anti-STAT1 antibody A00036-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-21

Question

We have observed staining in monkey epithelium of bronchus. Any tips? Is anti-STAT1 antibody supposed to stain epithelium of bronchus positively?

G. Anderson

Verified customer

Asked: 2016-12-22

Answer

From what I have seen in literature epithelium of bronchus does express STAT1. From what I have seen in Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-12-22

Question

Is this A00036-2 anti-STAT1 antibody reactive to the isotypes of STAT1?

A. Huang

Verified customer

Asked: 2016-03-04

Answer

The immunogen of A00036-2 anti-STAT1 antibody is E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-03-04

Question

We have tried in the past anti-STAT1 antibody for ICC on leukemic t-cell in the past. I am using monkey, and I plan to use the antibody for Flow Cytometry next. We need examining leukemic t-cell as well as epithelium of bronchus in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

T. Yang

Verified customer

Asked: 2015-01-08

Answer

I have checked the website and datasheets of our anti-STAT1 antibody and it seems that A00036-2 has been validated on monkey in both ICC and Flow Cytometry. Thus A00036-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in monkey even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-01-08

Question

Would anti-STAT1 antibody A00036-2 work for ICC with placenta testis?

C. Anderson

Verified customer

Asked: 2014-08-25

Answer

According to the expression profile of placenta testis, STAT1 is highly expressed in placenta testis. So, it is likely that anti-STAT1 antibody A00036-2 will work for ICC with placenta testis.

Boster Scientific Support

Answered: 2014-08-25

Conjugation

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A00036-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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