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- Table of Contents
FABP1 antibody (PB9586) performs well in IHC, WB and shows reactivity with Chicken, Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of liver FABP using anti-liver FABP antibody (PB9586).
liver FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-liver FABP Antibody (PB9586) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
liver FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-liver FABP Antibody (PB9586) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Designing a Western blot for FABP1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the FABP1 Western Blot GuideBundle Save
Anti-liver FABP/FABP1 Antibody Picoband®
FABP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Chicken, Human, Monkey, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
FABP1 antibody was positively detected by WB in:
human hepatocellular carcinoma tumor tissue (HCCT), chicken liver tissue, monkey liver tissue, rat liver tissue, rat small intestine tissue, rat RH35 whole cell, rat PC-12 whole cell, mouse liver tissue, mouse small intestine tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FABP1 antibody was positively detected by IHC in:
mouse intestine tissue, human intestinal cancer tissue, rat intestine tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey, Mouse, Rat, Chicken
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Chicken
Tested
Monkey
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-liver FABP/FABP1 Antibody Picoband®
View all FABP1 Antibodies
View all FABP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human liver FABP, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
14.2 kDa
Background of FABP1
Fatty acid binding protein 1, liver, also known as FABP1 or FABPL, is a human gene locating at 2p11. FABP1 encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind free fatty acids, their CoA derivatives, bilirubin, organic anions, and other small molecules. FABP1 and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metaboism. The liver form of FABP may be identical to the major liver protein-1 (Lvp-1), which is encoded by a gene situated within 1 cM of Ly-2.
Cite This Product
Anti-liver FABP/FABP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9586)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Mouse
94%
High Homology
Dog
90%
High Homology
Rat
87%
Moderate Homology
Cow
74%
Moderate Homology
Rabbit
71%
Moderate Homology
Sheep
71%
Moderate Homology
Zebrafish
71%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FABP1 antibody
Immunohistochemistry (IHC) protocol for FABP1 antibody
Publications
Application:
Species:
Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes
Published: 2021-04-02
Journal: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
Application: WB
Impact Factor: 4.29
Species: Bovine
PMID: 33819629
L-FABP T94A decreased fatty acid uptake and altered hepatic triglyceride and cholesterol accumulation in Chang liver cells stably transfected with L-FABP
Published: 2010-08-20
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 1.90
Species: Human
PMID: 20721681
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Product Promise
We promise Anti-liver FABP/FABP1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9586
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 29, 2026