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Product Info Summary
| SKU: | A07471-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-LPCAT2/Acyltransferase Like 1 Antibody
SKU/Catalog Number
A07471-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-LPCAT2/Acyltransferase Like 1 Antibody (Catalog# A07471-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LPCAT2/Acyltransferase Like 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07471-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human LPCAT2. Immunogen sequence location: 321-370
Reactive Species
A07471-1 is reactive to LPCAT2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
60.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07471-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from HT-29 cells using LPCAT2 antibody.
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Western blot analysis of lysates from K562 and HT-29 cells, using LPCAT2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-LPCAT2/Acyltransferase Like 1 Antibody (A07471-1)
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3 Customer Q&As for Anti-LPCAT2/Acyltransferase Like 1 Antibody
Question
I was wanting to use your anti-LPCAT2/Acyltransferase Like 1 antibody for WB for human fetal kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal kidney identification?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
As indicated on the product datasheet, A07471-1 anti-LPCAT2/Acyltransferase Like 1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
We are currently using anti-LPCAT2/Acyltransferase Like 1 antibody A07471-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-LPCAT2/Acyltransferase Like 1 antibody (A07471-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
Question
Will A07471-1 anti-LPCAT2/Acyltransferase Like 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Singh
Verified customer
Asked: 2015-07-08
Answer
It shows on the product datasheet, A07471-1 anti-LPCAT2/Acyltransferase Like 1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-08
