Product Info Summary
SKU: | PB10036 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-AKR1C1/C2 Antibody Picoband™
SKU/Catalog Number
PB10036
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AKR1C1/C2 Antibody Picoband™ catalog # PB10036. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKR1C1/C2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10036)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10036 is reactive to AKR1C2 in Human, Mouse, Rat
Applications
PB10036 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
36.735kDa
Background of AKR1C2
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HCCP tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mosue liver tissue lysates,
Lane 7: mosue kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C1/C2 antigen affinity purified polyclonal antibody (Catalog # PB10036) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C1/C2 at approximately 37 kDa. The expected band size for AKR1C1/C2 is at 37 kDa.
Click image to see more details
Figure 2. IF analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036).
AKR1C1/C2 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AKR1C1/C2 Antibody (PB10036) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of HepG2 cells using anti-AKR1C1/C2 antibody (PB10036).
Overlay histogram showing HepG2 cells stained with PB10036 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1C1/C2 Antibody (PB10036, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For AKR1C2 (Source: Uniprot.org, NCBI)
Gene Name
AKR1C2
Full Name
Aldo-keto reductase family 1 member C2
Weight
36.735kDa
Superfamily
aldo/keto reductase family
Alternative Names
aldo-keto reductase family 1 member C2; aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III); BABP; Chlordecone reductase homolog HAKRD; DD; DD-2; DD2DD/BABP; DDH2FLJ53800; Dihydrodiol dehydrogenase 2; Dihydrodiol dehydrogenase/bile acid-binding protein; EC 1.1.1,3-alpha-HSD3; EC 1.1.1.213; EC 1.3.1.20; HAKRDAKR1C-pseudo; HBAB; MCDR2; pseudo-chlordecone reductase; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II dihydrodiol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase AKR1C2 AKR1C-pseudo, BABP, DD, DD-2, DD/BABP, DD2, DDH2, HAKRD, HBAB, MCDR2, SRXY8, TDD aldo-keto reductase family 1 member C2 aldo-keto reductase family 1 member C2|3-alpha-HSD3|chlordecone reductase homolog HAKRD|dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III|pseudo-chlordecone reductase|testicular 17,20-desmolase deficiency|trans-1,2-dihydrobenzene-1,2-diol dehydrogenase|type II dihydrodiol dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKR1C2, check out the AKR1C2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKR1C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKR1C1/C2 Antibody Picoband™ (PB10036)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-AKR1C1/C2 Antibody Picoband™
Question
We are currently using anti-AKR1C1/C2 antibody PB10036 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
M. Krishna
Verified customer
Asked: 2020-02-10
Answer
The anti-AKR1C1/C2 antibody (PB10036) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
See below the WB image, lot number and protocol we used for prostate using anti-AKR1C1/C2 antibody PB10036. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
Do you have a BSA free version of anti-AKR1C1/C2 antibody PB10036 available?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AKR1C1/C2 antibody PB10036 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-16
Question
Will PB10036 anti-AKR1C1/C2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
It shows on the product datasheet, PB10036 anti-AKR1C1/C2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-30
Question
Does anti-AKR1C1/C2 antibody PB10036 work for WB with prostate?
N. Carter
Verified customer
Asked: 2014-10-09
Answer
According to the expression profile of prostate, AKR1C2 is highly expressed in prostate. So, it is likely that anti-AKR1C1/C2 antibody PB10036 will work for WB with prostate.
Boster Scientific Support
Answered: 2014-10-09
Question
I was wanting to use your anti-AKR1C1/C2 antibody for WB for rat prostate on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat prostate identification?
T. Edwards
Verified customer
Asked: 2014-08-05
Answer
You can see on the product datasheet, PB10036 anti-AKR1C1/C2 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-05