Anti-AKR1C1/C2 Antibody Picoband™

AKR1C2 antibody

Boster Bio Anti-AKR1C1/C2 Antibody Picoband™ catalog # PB10036. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10036
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-AKR1C1/C2 Antibody Picoband™

View all AKR1C2 Antibodies

SKU/Catalog Number

PB10036

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AKR1C1/C2 Antibody Picoband™ catalog # PB10036. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKR1C1/C2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10036)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10036 is reactive to AKR1C2 in Human, Mouse, Rat

Applications

PB10036 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

36.735kDa

Background of AKR1C2

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For AKR1C2 (Source: Uniprot.org, NCBI)

Gene Name

AKR1C2

Full Name

Aldo-keto reductase family 1 member C2

Weight

36.735kDa

Superfamily

aldo/keto reductase family

Alternative Names

aldo-keto reductase family 1 member C2; aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III); BABP; Chlordecone reductase homolog HAKRD; DD; DD-2; DD2DD/BABP; DDH2FLJ53800; Dihydrodiol dehydrogenase 2; Dihydrodiol dehydrogenase/bile acid-binding protein; EC 1.1.1,3-alpha-HSD3; EC 1.1.1.213; EC 1.3.1.20; HAKRDAKR1C-pseudo; HBAB; MCDR2; pseudo-chlordecone reductase; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II dihydrodiol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase AKR1C2 AKR1C-pseudo, BABP, DD, DD-2, DD/BABP, DD2, DDH2, HAKRD, HBAB, MCDR2, SRXY8, TDD aldo-keto reductase family 1 member C2 aldo-keto reductase family 1 member C2|3-alpha-HSD3|chlordecone reductase homolog HAKRD|dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III|pseudo-chlordecone reductase|testicular 17,20-desmolase deficiency|trans-1,2-dihydrobenzene-1,2-diol dehydrogenase|type II dihydrodiol dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKR1C2, check out the AKR1C2 Infographic

AKR1C2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKR1C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-AKR1C1/C2 Antibody Picoband™

Question

We are currently using anti-AKR1C1/C2 antibody PB10036 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?

M. Krishna

Verified customer

Asked: 2020-02-10

Answer

The anti-AKR1C1/C2 antibody (PB10036) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-10

Question

See below the WB image, lot number and protocol we used for prostate using anti-AKR1C1/C2 antibody PB10036. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-27

Question

Do you have a BSA free version of anti-AKR1C1/C2 antibody PB10036 available?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AKR1C1/C2 antibody PB10036 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-16

Question

Will PB10036 anti-AKR1C1/C2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-30

Answer

It shows on the product datasheet, PB10036 anti-AKR1C1/C2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-30

Question

Does anti-AKR1C1/C2 antibody PB10036 work for WB with prostate?

N. Carter

Verified customer

Asked: 2014-10-09

Answer

According to the expression profile of prostate, AKR1C2 is highly expressed in prostate. So, it is likely that anti-AKR1C1/C2 antibody PB10036 will work for WB with prostate.

Boster Scientific Support

Answered: 2014-10-09

Question

I was wanting to use your anti-AKR1C1/C2 antibody for WB for rat prostate on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat prostate identification?

T. Edwards

Verified customer

Asked: 2014-08-05

Answer

You can see on the product datasheet, PB10036 anti-AKR1C1/C2 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat prostate in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-08-05

Size

Conjugation

Total: $370

SKU:PB10036

In stock, 1 left.

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PB10036
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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