Skip to the beginning of the images gallery

ACSL4 antibody (A04372-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 19 Publications

Datasheet MSDS Request COA

a04372 2 acsl4 primary antibodies fcm testing 5

View All Images (7)

Flow Cytometry analysis of HepG2 cells using anti-FACL4/ACSL4 antibody (A04372-2).
Overlay histogram showing HepG2 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FACL4/ACSL4 Antibody (A04372-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-FACL4/ACSL4 Antibody Picoband®

ACSL4 antibody

19 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

ACSL4 antibody was positively detected by WB in:

human placenta tissue, human HepG2 whole cell, rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACSL4 antibody was positively detected by IHC in:

mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACSL4 antibody was positively detected by ICC/IF in:

A431 cell

ACSL4 antibody was positively detected by FC in:

HepG2 cell, U87 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Zebrafish

Predicted

Product Information

Product Name

Anti-FACL4/ACSL4 Antibody Picoband®
View all ACSL4 Antibodies

Form

Lyophilized

Uniprot

O60488

Gene ID

2182

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FACL4/ACSL4.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

79 kDa

Calculated Molecular Weight

79.2 kDa

Background of ACSL4

Long-chain-fatty-acid—CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Cite This Product

Anti-FACL4/ACSL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04372-2)

Protocols

Western Blot protocol for ACSL4 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACSL4 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ACSL4 antibody

Review Protocol

Flow Cytometry (FC) protocol for ACSL4 antibody

Review Protocol

Publications

Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics

Published: 2026-07-29

Journal: Frontiers in Pharmacology

Application: WB

Impact Factor: 5.40

Species: Mouse

PMID: 42591220

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-FACL4/ACSL4 Antibody Picoband®

ACSL4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics

Synergistic cardioprotection by liraglutide and deferoxamine against doxorubicin-induced cardiotoxicity via modulation of ACSL-4/Nrf-2/GPX-4 signaling

A supramolecular cordycepin hydrogel with spinal cord injury repair function

β-Caryophyllene confers protection against type 2 diabetic osteoporosis by blocking ferroptosis via the AMPK/Nrf2 pathway

Regulating the GTP cyclohydrolase 1/tetrahydrobiopterin axis in the distal tubule inhibits ferroptosis and alleviates crush syndrome-related acute kidney injury in mice

Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway

OSGEP, A Negative Ferroptotic Regulator, Alleviates Cerebral Ischemia–Reperfusion Injury Through Modulating m6A Methylation of GPX4 mRNA

Diselenide Nanogels Modulate Mitochondrial Function and Mitigate Oxidative Stress in Cardiomyocytes for Enhanced Cardiac Repair

MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import

Delay the progression of glucocorticoid-induced osteoporosis: Fraxin targets ferroptosis via the Nrf2/GPX4 pathway

Quercetin attenuates cerebral ischemic injury by inhibiting ferroptosis via Nrf2/HO-1 signaling pathway

Characterization of PKCα-rutin interactions and its application as a treatment strategy for PAH by inhibiting ferroptosis

Porphyromonas gingivalis as a promotor in the development of the alcoholic liver disease via ferroptosis

Paeoniae Radix Rubra extract attenuates cerebral ischemia injury by inhibiting ferroptosis and activating autophagy through the PI3K/Akt signalling pathway

Inhibition of MALT1 reduces ferroptosis in rat hearts following ischemia/reperfusion via enhancing the Nrf2/SLC7A11 pathway

Porphyromonas gingivalis is a risk factor for the development of nonalcoholic fatty liver disease via ferroptosis

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway.

Old targets, new strategy: Apigenin-7-O-β-d-(-6″-p-coumaroyl)-glucopyranoside prevents endothelial ferroptosis and alleviates intestinal ischemia-reperfusion injury through HO-1 and MAO-B inhibition

Scutellaria barbata Inhibits Hepatocellular Carcinoma Tumorigenicity by Inducing Ferroptosis of Hepatocellular Carcinoma Cells

Review

Have you used Anti-FACL4/ACSL4 Antibody Picoband®?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality