Anti-FACL4/ACSL4 Antibody Picoband™

FACL4 antibody

Boster Bio Anti-FACL4/ACSL4 Antibody Picoband™ catalog # A04372-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04372-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-FACL4/ACSL4 Antibody Picoband™

View all FACL4 Antibodies

SKU/Catalog Number

A04372-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FACL4/ACSL4 Antibody Picoband™ catalog # A04372-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FACL4/ACSL4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04372-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FACL4/ACSL4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04372-2 is reactive to ACSL4 in Human, Mouse, Rat

Applications

A04372-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

79 kDa

Calculated molecular weight

79.188kDa

Background of FACL4

Long-chain-fatty-acid—CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACSL4 (Source: Uniprot.org, NCBI)

Gene Name

ACSL4

Full Name

Long-chain-fatty-acid--CoA ligase 4

Weight

79.188kDa

Superfamily

ATP-dependent AMP-binding enzyme family

Alternative Names

ACS4mental retardation, X-linked 68; acyl-CoA synthetase 4; acyl-CoA synthetase long-chain family member 4; EC 6.2.1.3; FACL4long-chain 4; LACS 4; LACS4MRX68; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; long-chain-fatty-acid--CoA ligase 4; mental retardation, X-linked 63; MRX63 ACSL4 ACS4, FACL4, LACS4, MRX63, MRX68 acyl-CoA synthetase long chain family member 4 long-chain-fatty-acid--CoA ligase 4|acyl-CoA synthetase 4|arachidonate--CoA ligase|fatty-acid-Coenzyme A ligase, long-chain 4|lignoceroyl-CoA synthase|long-chain acyl-CoA synthetase 4|long-chain fatty-acid-Coenzyme A ligase 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACSL4, check out the ACSL4 Infographic

ACSL4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACSL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-FACL4/ACSL4 Antibody Picoband™

Question

We are currently using anti-FACL4/ACSL4 antibody A04372-2 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

The anti-FACL4/ACSL4 antibody (A04372-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-25

Size

Conjugation

Total: $370

SKU:A04372-2

In stock, 1 left.

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A04372-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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