Anti-Anterior Gradient 2/AGR2 Antibody Picoband™

AG-2/AGR2 antibody

Boster Bio Anti-Anterior Gradient 2/AGR2 Antibody Picoband™ catalog # A02922-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02922-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Anterior Gradient 2/AGR2 Antibody Picoband™

View all AG-2/AGR2 Antibodies

SKU/Catalog Number

A02922-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Anterior Gradient 2/AGR2 Antibody Picoband™ catalog # A02922-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Anterior Gradient 2/AGR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02922-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Anterior Gradient 2 recombinant protein (Position: R21-L175). Human Anterior Gradient 2 shares 93.5% amino acid (aa) sequence identity with mouse Anterior Gradient 2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02922-2 is reactive to AGR2 in Human, Mouse, Rat

Applications

A02922-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

19.979kDa

Background of AG-2/AGR2

Anterior gradient protein 2 homolog (AGR-2), also known as secreted cement gland protein XAG-2 homolog, is a protein that in humans is encoded by the AGR2 gene. Anterior gradient homolog 2 was originally discovered in Xenopus laevis. In Xenopus AGR2 plays a role in cement gland differentiation, but in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response, cell migration, and cell transformation. However, there have been other observations that AGR2 can repress growth and proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For AGR2 (Source: Uniprot.org, NCBI)

Gene Name

AGR2

Full Name

Anterior gradient protein 2 homolog

Weight

19.979kDa

Superfamily

AGR family

Alternative Names

AG2; AG-2; AG2member 17; AGR2; anterior gradient 2 homolog (Xenopus laevis); anterior gradient homolog 2 (Xenopus laevis); GOB-4; hAG-2; HPC8; PDIA17; secreted cement gland homolog AGR2 AG-2, AG2, GOB-4, HAG-2, HEL-S-116, HPC8, PDIA17, XAG-2 anterior gradient 2, protein disulphide isomerase family member anterior gradient protein 2 homolog|anterior gradient homolog 2|epididymis secretory protein Li 116|protein disulfide isomerase family A, member 17|secreted cement gland homolog|secreted cement gland protein XAG-2 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AGR2, check out the AGR2 Infographic

AGR2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AGR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Anterior Gradient 2/AGR2 Antibody Picoband™

Question

We are currently using anti-Anterior Gradient 2/AGR2 antibody A02922-2 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

The anti-Anterior Gradient 2/AGR2 antibody (A02922-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of sigmoid colon using anti-Anterior Gradient 2/AGR2 antibody A02922-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-07-27

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-27

Question

I see that the anti-Anterior Gradient 2/AGR2 antibody A02922-2 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-12

Question

I was wanting to use your anti-Anterior Gradient 2/AGR2 antibody for IHC for rat mucosa of sigmoid colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat mucosa of sigmoid colon identification?

M. Rodriguez

Verified customer

Asked: 2017-01-25

Answer

It shows on the product datasheet, A02922-2 anti-Anterior Gradient 2/AGR2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mucosa of sigmoid colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-01-25

Size

Conjugation

Total: $370

SKU:A02922-2

Backordered.

Lead time for this item is typically 2-3 weeks

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A02922-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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