Anti-Lysozyme LYZ Rabbit Monoclonal Antibody

Lysozyme antibody

Boster Bio Anti-Lysozyme LYZ Rabbit Monoclonal Antibody catalog # M01811. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: M01811
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Lysozyme LYZ Rabbit Monoclonal Antibody

View all Lysozyme Antibodies

SKU/Catalog Number

M01811

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Lysozyme LYZ Rabbit Monoclonal Antibody catalog # M01811. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lysozyme LYZ Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01811)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EBO-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Lysozyme

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01811 is reactive to LYZ in Human, Mouse, Rat

Applications

M01811 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

16.537kDa

Background of Lysozyme

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20

Validation Images & Assay Conditions

Gene/Protein Information For LYZ (Source: Uniprot.org, NCBI)

Gene Name

LYZ

Full Name

Lysozyme C

Weight

16.537kDa

Superfamily

glycosyl hydrolase 22 family

Alternative Names

EC 3.2.1.17; lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C; lysozyme C; Lysozyme; LZM; renal amyloidosis LYZ LYZF1, LZM lysozyme lysozyme C|1,4-beta-N-acetylmuramidase C|c-type lysozyme|lysozyme F1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LYZ, check out the LYZ Infographic

LYZ infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LYZ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01811 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cuiling Guo,Dandan Guo,Liu Fang,Tingting Sang,Jianjun Wu,Chengjie Guo,Yujie Wang,Ying Wang,Chaojie Chen,Jiajun Chen,Rong Chen,Xingya Wang,Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon,Carbohydrate Polymers,Volume 267,2021,118231,ISSN 0144-8617, https:// doi.org/10.1016/j.carbpol.2021.118231.
Species: Mouse
M01811 usage in article: APP:WB, SAMPLE:RAW264.7 CELL AND COLON TISSUE, DILUTION:1:1000

Sang T,Guo C,Guo D,Wu J,Wang Y,Wang Y,Chen J,Chen C,Wu K,Na K,Li K,Fang L,Guo C,Wang X. Suppression of obesity and inflammation by polysaccharide from sporoderm-broken spore of Ganoderma lucidum via gut microbiota regulation. Carbohydr Polym.2021 Mar 15;2
Species: Mouse
M01811 usage in article: APP:WB, SAMPLE:SERUM, DILUTION:1:1000

Sang T,Guo C,Guo D,Wu J,Wang Y,Wang Y,Chen J,Chen C,Wu K,Na K,Li K,Fang L,Guo C,Wang X. Suppression of obesity and inflammation by polysaccharide from sporoderm-broken spore of Ganoderma lucidum via gut microbiota regulation. Carbohydr Polym.2021 Mar 15;2
Species: Mouse
M01811 usage in article: APP:WB, SAMPLE:SERUM, DILUTION:1:1000

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16 Customer Q&As for Anti-Lysozyme LYZ Rabbit Monoclonal Antibody

Question

Does M01811 anti-Lysozyme Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

You can see on the product datasheet, M01811 anti-Lysozyme Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-18

Question

We have observed staining in mouse colon. Any tips? Is anti-Lysozyme Rabbit Monoclonal antibody supposed to stain colon positively?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

From literature colon does express LYZ. From Uniprot.org, LYZ is expressed in nasal cavity mucosa, colon, urine, milk, tear, among other tissues. Regarding which tissues have LYZ expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Milk, Pubmed ID: 5168859, 11946553
Tear, Pubmed ID: 25946035
Urine, Pubmed ID: 5284421, 11946554

Boster Scientific Support

Answered: 2020-01-08

Question

Our team were happy with the WB result of your anti-Lysozyme Rabbit Monoclonal antibody. However we have been able to see positive staining in nasal cavity mucosa secreted. using this antibody. Is that expected? Could you tell me where is LYZ supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

According to literature, nasal cavity mucosa does express LYZ. Generally LYZ expresses in secreted. Regarding which tissues have LYZ expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Milk, Pubmed ID: 5168859, 11946553
Tear, Pubmed ID: 25946035
Urine, Pubmed ID: 5284421, 11946554

Boster Scientific Support

Answered: 2019-12-02

Question

We have tried in the past anti-Lysozyme Rabbit Monoclonal antibody for IP on tear in the past. I am using human, and We want to use the antibody for IHC next. My lab would like examining tear as well as milk in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

I looked at the website and datasheets of our anti-Lysozyme Rabbit Monoclonal antibody and it seems that M01811 has been validated on human in both IP and IHC. Thus M01811 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-22

Question

I was wanting to use to test anti-Lysozyme Rabbit Monoclonal antibody M01811 on mouse colon for research purposes, then I may be interested in using anti-Lysozyme Rabbit Monoclonal antibody M01811 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

The products we sell, including anti-Lysozyme Rabbit Monoclonal antibody M01811, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-28

Question

Do you have a BSA free version of anti-Lysozyme Rabbit Monoclonal antibody M01811 available?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Lysozyme Rabbit Monoclonal antibody M01811 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-25

Question

See attached the WB image, lot number and protocol we used for colon using anti-Lysozyme Rabbit Monoclonal antibody M01811. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-16

Question

Is a blocking peptide available for product anti-Lysozyme Rabbit Monoclonal antibody (M01811)?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We do provide the blocking peptide for product anti-Lysozyme Rabbit Monoclonal antibody (M01811). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-07

Question

Can you help my question with product M01811, anti-Lysozyme Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01811 anti-Lysozyme Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-31

Question

Is this M01811 anti-Lysozyme Rabbit Monoclonal antibody reactive to the isotypes of LYZ?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The immunogen of M01811 anti-Lysozyme Rabbit Monoclonal antibody is A synthesized peptide derived from human Lysozyme . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-24

Question

Would anti-Lysozyme Rabbit Monoclonal antibody M01811 work for ICC with colon?

A. Krishna

Verified customer

Asked: 2017-10-26

Answer

According to the expression profile of colon, LYZ is highly expressed in colon. So, it is likely that anti-Lysozyme Rabbit Monoclonal antibody M01811 will work for ICC with colon.

Boster Scientific Support

Answered: 2017-10-26

Question

I was wanting to use your anti-Lysozyme Rabbit Monoclonal antibody for ICC for mouse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon identification?

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

You can see on the product datasheet, M01811 anti-Lysozyme Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-11

Question

I see that the anti-Lysozyme Rabbit Monoclonal antibody M01811 works with ICC, what is the protocol used to produce the result images on the product page?

W. Miller

Verified customer

Asked: 2017-10-10

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-10-10

Question

Our lab want to know about using your anti-Lysozyme Rabbit Monoclonal antibody for cellular protein metabolic process studies. Has this antibody been tested with western blotting on mouse kidney? We would like to see some validation images before ordering.

L. Krishna

Verified customer

Asked: 2014-07-29

Answer

I appreciate your inquiry. This M01811 anti-Lysozyme Rabbit Monoclonal antibody is tested on k562 cell lysate, mouse kidney. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-07-29

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-Lysozyme Rabbit Monoclonal antibody M01811. Let me know if you need anything else.

M. Carter

Verified customer

Asked: 2014-03-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-03

Question

We are currently using anti-Lysozyme Rabbit Monoclonal antibody M01811 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?

C. Bhatt

Verified customer

Asked: 2013-08-09

Answer

The anti-Lysozyme Rabbit Monoclonal antibody (M01811) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-09

Order DetailsPrice
M01811

100μL

$370
M01811-30ul

30μL sample

$99
M01811-carrier-free

Carrier Free

$370

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