Anti-MAD1/MAD1L1 Antibody Picoband™

Boster Bio Anti-MAD1/MAD1L1 Antibody Picoband™ catalog # PB9262. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9262
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-MAD1/MAD1L1 Antibody Picoband™

See all MAD1L1/MAD1 products

SKU/Catalog Number

PB9262

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAD1/MAD1L1 Antibody Picoband™ catalog # PB9262. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAD1/MAD1L1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9262)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9262 is reactive to MAD1L1 in Human, Mouse, Rat

Applications

PB9262 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry, 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MAD1L1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y6D9

Gene ID

8379

Gene Name

MAD1L1

Full Name

Mitotic spindle assembly checkpoint protein MAD1

Weight

83.067kDa

Superfamily

MAD1 family

Alternative Names

hMAD1; HsMAD1; MAD1 mitotic arrest deficient-like 1 (yeast); MAD1; MAD1L1; MAD1-like protein 1; MAD1yeast, homolog-like 1; Mitotic checkpoint MAD1 protein homolog; PIG9; Tax-binding protein 181; TP53I9; tumor protein p53 inducible protein 9; TXBP181; TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1 MAD1L1 MAD1, PIG9, TP53I9, TXBP181 mitotic arrest deficient 1 like 1 mitotic spindle assembly checkpoint protein MAD1|MAD1 mitotic arrest deficient like 1|MAD1-like protein 1|mitotic arrest deficient 1-like protein 1|mitotic checkpoint MAD1 protein homolog|mitotic-arrest deficient 1, yeast, homolog-like 1|tax-binding protein 181|tumor protein p53 inducible protein 9

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAD1L1, check out the MAD1L1 Infographic

MAD1L1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAD1L1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB9262

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5 Customer Q&As for Anti-MAD1/MAD1L1 Antibody Picoband™

Question

See below the WB image, lot number and protocol we used for embryonic kidney using anti-MAD1/MAD1L1 antibody PB9262. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-10

Question

We are currently using anti-MAD1/MAD1L1 antibody PB9262 for mouse tissue, and we are content with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

The anti-MAD1/MAD1L1 antibody (PB9262) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-03

Question

I see that the anti-MAD1/MAD1L1 antibody PB9262 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-03

Question

Would PB9262 anti-MAD1/MAD1L1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

You can see on the product datasheet, PB9262 anti-MAD1/MAD1L1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-01

Question

My question regarding product PB9262, anti-MAD1/MAD1L1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9262 anti-MAD1/MAD1L1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-19

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

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PB9262
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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