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Product Info Summary
| SKU: | PB9665 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Monoamine Oxidase B/MAOB Antibody Picoband®
SKU/Catalog Number
PB9665
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Monoamine Oxidase B/MAOB Antibody Picoband® catalog # PB9665. Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Monoamine Oxidase B/MAOB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9665)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MAOB, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9665 is reactive to MAOB in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
58.8 kDa
Background of MAOB
MAOB (MONOAMINE OXIDASE B), also called MAO, BRAIN, AMINE OXIDASE (FLAVIN-CONTAINING) B, is a protein that in humans is encoded by the MAOB gene. MAOB is a member of the flavin monoamine oxidase family. And it is mapped on Xp11.3. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. Like MAOA, it also degrades dopamine. MAO-B is involved in the breakdown of dopamine, a neurotransmitter implicated in reinforcing and motivating behaviors as well as movement. MAO-B inhibition is, therefore, associated with enhanced activity of dopamine, as well as with decreased production of hydrogen peroxide, a source of reactive oxygen species.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9665 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Positive Control
WB: human SiHa whole cell, human HepG2 whole cell, human Hela whole cell, human placenta tissue, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue
IHC: human colon cancer tissue
IP: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of MAOB using anti-MAOB antibody (PB9665).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (PB9665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
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IHC analysis of MAOB using anti-MAOB antibody (PB9665).
MAOB was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAOB Antibody (PB9665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of MAOB using anti-MAOB antibody (PB9665).
MAOB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAOB Antibody (PB9665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunoprecipitating (IP) MAOB in Hela whole cell lysate.
Western blot analysis of MAOB using anti-MAOB antibody (PB9665);
Lane 1: Hela whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-MAOB antibody in Hela whole cell lysate;
Lane 3: anti-MAOB antibody (2μg) + Hela whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (PB9665) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
Specific Publications For Anti-Monoamine Oxidase B/MAOB Antibody Picoband® (PB9665)
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2 Customer Q&As for Anti-Monoamine Oxidase B/MAOB Antibody Picoband®
Question
What is the detailed/specific WB protocol used to produce images for the PB9665 antibody?
Verified customer
Asked: 2019-11-14
Answer
For the Anti-Monoamine Oxidase B/MAOB Antibody Picoband PB9665 WB images, Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC4 antigen affinity purified polyclonal antibody (Catalog no. PB9665) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody(Catalog no. BA1054) at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog no. EK1002) with Tanon 5200 system.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-Monoamine Oxidase B/MAOB antibody PB9665 for monkey tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The anti-Monoamine Oxidase B/MAOB antibody (PB9665) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-23
