Anti-Cystathionase/CTH Antibody Picoband™
Rabbit IgG polyclonal antibody for Cystathionine gamma-lyase(CTH) detection. Tested with WB in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-Cystathionase/CTH Antibody Picoband™ Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Cystathionase/CTH Antibody Picoband™
See all Cystathionase primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CYSTATHIONASE/CTH detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human;Mouse;Rat. CYSTATHIONASE/CTH information: Molecular Weight: 44508 MW; Subcellular Localization: Cytoplasm.|
|Cite This Product||Anti-Cystathionase/CTH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9494)|
|Similar Products From
|Anti-Cystathionase/CTH Antibody Picoband™ may replace the following items: sc 135203|sc 374249|sc 365382|sc 365381|sc 131905|sc 101924.|
|Reactivity/Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||E.coli-derived human Cystathionase recombinant protein (Position: D181-H398). Human Cystathionase shares 85.8% amino acid (aa) sequence identity with both mouse and rat Cystathionase.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CTH (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1491|
|Species Of This Entry||Human|
|Protein Name||Cystathionine gamma-lyase|
|Superfamily||trans-sulfuration enzymes family|
|Alternative Names||Cystathionase|cystathionase (cystathionine gamma-lyase); cystathionine gamma-lyase; cysteine desulfhydrase; EC 4.4.1; EC 22.214.171.124; gamma-cystathionase; homoserine deaminase; homoserine dehydratase; MGC9471|
|Post Tranlational Modifications||Cleavage; Methylation; Phosphorylation; Reduction; Nitration; Carboxylation; Biotinylation; Amination; Oxidation|
|Gene Location||1p31.1, on Chromasome 1, gene sequence: NC_000001.11 (70411218..70441949)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CTH (Source: Uniprot.org, NCBI)
|Protein Function||Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.|
|Interacting Proteins||GUCD1; WDYHV1|
|Related Diseases||Fabry Disease; Headache; Tension Headache; Neoplasms; Malignant Neoplasms; Migraine Disorders; 5,10-methylenetetrahydrofolate Reductase Deficiency; Chronic Tension-type Headache; Pain; Carcinoma; Gamma-cystathionase Deficiency; Depressive Disorder; Lymphoma; Hypertrophy; Hyperhomocysteinemia; Stress, Psychological; Impaired Cognition; Hypertensive Disease; Heart Failure; Mild Cognitive Disorder|
|Related Pathways||Cell Growth; Pathogenesis; Transport; Immune Response; Reflex; Cell Cycle; Cell Differentiation; Localization; Coagulation; Excretion; Quorum Sensing; Stem Cell Differentiation; Hypersensitivity; M Phase; Serotonin Uptake; Cell Division; Flight; Chemotaxis; Sensitization; Phagocytosis|
|Background||Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and α-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.|
|Scientific References||PMID: 1339280 by Lu Y., et al. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase.|
PMID: 10212249 by Steegborn C., et al. Kinetics and inhibition of recombinant human cystathionine gamma- lyase. Toward the rational control of transsulfuration.
Our Boster Quality Guarantee for Anti-Cystathionase/CTH Antibody Picoband™ covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: K562 whole cell lysates,
lane 2: HepG2 whole cell lysates,
lane 3: RH35 whole cell lysates,
lane 4: rat liver tissue lysates,
lane 5: rat kidney tissue lysates,
lane 6: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 5
|Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway||PubMed ID 29393353|
|Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats||PubMed ID 26378818|
|Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis||PubMed ID 26668593|
|Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats||PubMed ID 26798657|
|High C-X-C motif chemokine 5 expression is associated with malignant phenotypes of prostate cancer cells via autocrine and paracrine pathways||PubMed ID 29749439|
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Note: sample size takes longer to process, average 5-7 days.
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