Click image to see more details
Product Info Summary
| SKU: | PB9494 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cystathionase/CTH Antibody Picoband®
SKU/Catalog Number
PB9494
PB0516 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cystathionase/CTH Antibody Picoband® catalog # PB9494. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystathionase/CTH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9494)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cystathionase recombinant protein (Position: D181-H398). Human Cystathionase shares 85.8% amino acid (aa) sequence identity with both mouse and rat Cystathionase.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9494 is reactive to CTH in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.5 kDa
Background of CTH
Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and α-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9494 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: K562 whole cell, HepG2 whole cell,, RH35 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: K562 whole cell lysates,
lane 2: HepG2 whole cell lysates,
lane 3: RH35 whole cell lysates,
lane 4: rat liver tissue lysates,
lane 5: rat kidney tissue lysates,
lane 6: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
Specific Publications For Anti-Cystathionase/CTH Antibody Picoband® (PB9494)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cystathionase/CTH Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Cystathionase/CTH Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Cystathionase/CTH Antibody Picoband®
Question
We are currently using anti-Cystathionase/CTH antibody PB9494 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-Cystathionase/CTH antibody (PB9494) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
