|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-LRP5 Antibody|
|Description||Rabbit IgG polyclonal antibody for Low-density lipoprotein receptor-related protein 5(LRP5) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-LRP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2273)|
|Replacement Item||This antibody may replace the following items: sc-21389|sc-134463|sc-21390|sc-390267|sc-514713 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5(1201-1215aa IHAVEEVSLEEFSAH), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Low-density lipoprotein receptor-related protein 5|
|Molecular Weight||179145 MW|
|Protein Function||Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. .|
|Tissue Specificity||Widely expressed, with the highest level of expression in the liver and in aorta. .|
|Sequence Similarities||Belongs to the LDLR family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein. Endoplasmic reticulum . Chaperoned to the plasma membrane by MESD. .|
|Alternative Names||Low-density lipoprotein receptor-related protein 5;LRP-5;LRP5;LR3, LRP7;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| signal transduction|cytoskeleton / ecm|extracellular matrix|structures|bone| neuroscience|sensory system|visual system| stem cells|signaling pathways|wnt|surface molecules| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|cancer||
Background for Low-density lipoprotein receptor-related protein 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LRP5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LRP5 Antibody Images
Click the images to enlarge.
All lanes: Anti-LRP5(PA2273) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,