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- Table of Contents
LRP5 antibody (PA2273) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-LRP5 antibody, PA2273, All Western blotting
All lanes: Anti-LRP5(PA2273) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
All lanes: Anti-LRP5(PA2273) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
Designing a Western blot for LRP5? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the LRP5 Western Blot GuideBundle Save
Anti-LRP5 Antibody Picoband®
LRP5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
LRP5 antibody was positively detected by WB in:
Rat Liver Tissue, Mouse Liver Tissue, NIH Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat, Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Mouse
Tested
Human
Predicted
Monkey
Predicted
Dog
Predicted
Product Information
Product Name
Anti-LRP5 Antibody Picoband®
View all LRP5 Antibodies
View all LRP5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
179 kDa
Calculated Molecular Weight
179.1 kDa
Background of LRP5
Low-density lipoprotein receptor-related protein 5 is a protein that in humans is encoded by the LRP5 gene. It is mapped to 11q13.2. LRP5 is a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with diabetes mellitus type 1 in humans. This protein plays a key role in skeletal homeostasis. In addition to that, the binding of axin to LRP5 is an important part of the Wnt signal transduction pathway, and it also acts as a target for the inhibitory effects of Dickkopf, another developmental protein, on Wnt signaling.
Cite This Product
Anti-LRP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2273)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Monkey
100%
High Homology
Dog
93%
High Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LRP5 antibody
Publications
Application:
Species:
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway
Published: 2018-12-11
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 5.50
Species: Rat,Human
PMID: 30562742
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Product Promise
We promise Anti-LRP5 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PA2273
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026