Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
Customers Who Bought This Also Bought
Anti-MAP2 Antibody Picoband™
Boster Bio Anti-MAP2 Antibody Picoband™ catalog # A01201. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01201)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human MAP2 recombinant protein (Position: Q64-L133).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01201 is reactive to MAP2 in Human, Mouse, Rat
A01201 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280KD. The expected band size for MAP2 is at 199KD.
Gene/Protein Information For MAP2 (Source: Uniprot.Org, NCBI)
Microtubule-associated protein 2
DKFZp686I2148; MAP2; MAP-2; MAP2A; MAP2B; MAP2C; microtubule-associated protein 2 MAP2 MAP-2A, MAP2B, MAP2C, MAP2 microtubule associated protein 2 microtubule-associated protein 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MAP2, check out the MAP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01201 has been cited in 20 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effect of acetylcholine-like biomimetic polymers on neuronal growth
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Yang H,Zhang C.Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.Neurosci Lett. 2021 Feb 11:135714.doi:10.1016/j.neulet.2021.135714.Epub ahead of print.PMID:33582188.
A01201 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury %u2026
Nitrobenzylthioinosine mimics adenosine to attenuate the epileptiform discharge of hippocampal neurons from epileptic rats
Association of RASgrf1 methylation with epileptic seizures
Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a
Neuroprotective effects of leonurine against oxygen?glucose deprivation by targeting Cx36/CaMKII in PC12 cells
Lithium promotes DNA stability and survival of ischemic retinal neurocytes by upregulating DNA ligase IV
Establishment of Lipofection Protocol for Efficient miR-21 Transfection into Cortical Neurons In Vitro
Activation of the macroautophagic system in scrapie-infected experimental animals and human genetic prion diseases
Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke
Brca1 Is Upregulated by 5-Aza-CdR and Promotes DNA Repair and Cell Survival, and Inhibits Neurite Outgrowth in Rat Retinal Neurons
The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia
Liu L, Lai YJ, Zhao LG, Chen GJ. Exp Ther Med. 2017 Dec;14(6):5751-5756. doi: 10.3892/etm.2017.5289. Epub 2017 Oct 11. Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice
Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.
Chen Db, Feng L, Lin Xp, Zhang W, Li Fr, Liang Xl, Li Xh. Plos One. 2012;7(5):E37709. Doi: 10.1371/Journal.Pone.0037709. Epub 2012 May 21. Penicillamine Increases Free Copper And Enhances Oxidative Stress In The Brain Of Toxic Milk Mice.
Chen P, Hu H, Chen Z, Cai X, Zhang Z, Yang Y, Yu N, Zhang J, Xia L, Ge J, Yu K, Zhuang J. Plos One. 2014 Jun 11;9(6):E99371. Doi: 10.1371/Journal.Pone.0099371. Ecollection 2014. Brca1 Silencing Is Associated With Failure Of Dna Repairing In Retina...
Wang D, Ren M, Guo J, Yang G, Long X, Hu R, Shen W, Wang X, Zeng K. Plos One. 2014 Dec 23;9(12):E115801. Doi: 10.1371/Journal.Pone.0115801. Ecollection 2014. The Inhibitory Effects Of Npas4 On Seizures In Pilocarpine-Induced Epileptic Rats.
Sun X, Jiang R, Zhang Y, Chen M, Xiang P, Qi Y, Gao Q, Huang B, Ge J. Mol Vis. 2009 Dec 2;15:2503-14. Gene Expression And Differentiation Characteristics In Mice E13.5 And E17.5 Neural Retinal Progenitors.
Li T, Mo X, Jiang Z, He W, Lu W, Zhang H, Zhang J, Zeng L, Yang B, Xiao H, Hu Z. Oxid Med Cell Longev. 2012;2012:945071. Doi: 10.1155/2012/945071. Epub 2012 Oct 12. Study Of ??b-Crystallin Expression In Gerbil Bcao Model Of Transient Global Cerebr...
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-MAP2 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-MAP2 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question