Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PI3 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Product Info Summary

SKU: EZ1117
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Trappin-2/Elafin/Skalp products

SKU/Catalog Number

EZ1117

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PI3 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1117)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-Q117

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1117 is reactive to PI3 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1117 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1117-CA Rabbit anti- human PI3 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1117-DA Biotinylated goat anti- human PI3 polyclonal antibody (Detection Antibody) 500 µl, 3.3 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1117-ST Lyophilized recombinant human PI3 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.010.0730.1480.2710.5750.9661.7892.233

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)22565713342306451742
Standard deviation12.632.257.3617.0239.99113.23
CV (%)5.6%4.9%4.3%7.4%6.2%6.5%

Gene/Protein Information For PI3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P19957

Gene ID

5266

Gene Name

PI3

Full Name

Elafin

Weight

12.27kDa

Alternative Names

cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin PI3 ESI, SKALP, WAP3, WFDC14, cementoin peptidase inhibitor 3 elafin|PI-3|WAP four-disulfide core domain 14|WAP four-disulfide core domain protein 14|elastase-specific inhibitor|peptidase inhibitor 3, skin-derived|pre-elafin|protease inhibitor 3, skin-derived (SKALP)|protease inhibitor WAP3|skin-derived antileukoproteinase|trappin-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PI3, check out the PI3 Infographic

PI3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PI3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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