|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MAP2 Antibody (Monoclonal, HM-2)|
|Description||Mouse IgG monoclonal antibody for MAP2, microtubule-associated protein 2 (MAP2) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-MAP2 Antibody (Monoclonal, HM-2) HM-2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1057)|
|Replacement Item||This antibody may replace the following items: sc-12012|sc-135979|sc-166773|sc-166826|sc-166827|sc-20172|sc-32791|sc-365204|sc-365637|sc-365959|sc-390543|sc-49450|sc-49451|sc-49452|sc-5357|sc-5359|sc-56561|sc-67234|sc-74419|sc-74420|sc-74421|sc-74422|sc-80012|sc-80013 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Rat brain microtubule-associated proteins(MAPs).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Microtubule-associated protein 2|
|Molecular Weight||202411 MW|
|Protein Function||The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.|
|Sequence Similarities||Contains 3 Tau/MAP repeats.|
|Subcellular Localization||Cytoplasm, cytoskeleton .|
|Alternative Names||Microtubule-associated protein 2;MAP-2;Map2;Mtap2;|
|Research Areas|||neuroscience|cell type marker|neuron marker|soma marker| signal transduction|cytoskeleton / ecm|cytoskeleton|microtubules|mt associated proteins|map| neuroscience|dendrite marker| stem cells|neural stem cells|neuron restricted lineage||
Background for Microtubule-associated protein 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MAP2 Antibody (Monoclonal, HM-2) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Western blot, 0.5-2μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MAP2 Antibody (Monoclonal, HM-2) Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue