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Product Info Summary
| SKU: | A10230-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Uroplakin Ib/UPIb Antibody Picoband®
SKU/Catalog Number
A10230-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Uroplakin Ib/UPIb Antibody Picoband® catalog # A10230-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Uroplakin Ib/UPIb Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10230-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Uroplakin Ib/UPIb recombinant protein (Position: I107-Q216).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A10230-1 is reactive to UPK1B in Human, Mouse, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
29.6 kDa
Background of UPK1B
Uroplakin 1B, also known as UPK1B, is a protein which in humans is encoded by the UPK1B gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The use of alternate polyadenylation sites has been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10230-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human SK-OV-3 whole cell, rat stomach tissue, mouse stomach tissue
Validation Images & Assay Conditions
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Western blot analysis of Uroplakin Ib/UPIb using anti-Uroplakin Ib/UPIb antibody (A10230-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: mouse stomach tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uroplakin Ib/UPIb antigen affinity purified polyclonal antibody (Catalog # A10230-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uroplakin Ib/UPIb at approximately 30KD. The expected band size for Uroplakin Ib/UPIb is at 30KD.
Specific Publications For Anti-Uroplakin Ib/UPIb Antibody Picoband® (A10230-1)
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