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Product Info Summary
| SKU: | A10600-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Med18 Antibody
SKU/Catalog Number
A10600-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Med18 Antibody catalog # A10600-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Med18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10600-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MED18. AA range:70-119
Reactive Species
A10600-1 is reactive to MED18 in Human, Mouse, Rat
Calculated molecular weight
23.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10600-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Med18 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COLO and Jurkat cells, using MED18 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Med18 Antibody (A10600-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Med18 Antibody
Question
I was wanting to use your anti-Med18 antibody for WB for rat blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat blood identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It shows on the product datasheet, A10600-1 anti-Med18 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
Does anti-Med18 antibody A10600-1 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
According to the expression profile of blood, MED18 is highly expressed in blood. So, it is likely that anti-Med18 antibody A10600-1 will work for WB with blood.
Boster Scientific Support
Answered: 2019-12-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Med18 antibody A10600-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-02
Question
Can you help my question with product A10600-1, anti-Med18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Banerjee
Verified customer
Asked: 2016-08-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10600-1 anti-Med18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-24
Question
Is this A10600-1 anti-Med18 antibody reactive to the isotypes of MED18?
S. Evans
Verified customer
Asked: 2015-04-29
Answer
The immunogen of A10600-1 anti-Med18 antibody is Synthesized peptide derived from the Internal region of human Med18. at AA rangle: 40-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-29
