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Product Info Summary
| SKU: | A04895 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Smoothelin/SMTN Antibody Picoband®
SKU/Catalog Number
A04895
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smoothelin/SMTN Antibody Picoband® catalog # A04895. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smoothelin/SMTN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04895)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Smoothelin, which shares 97.3% amino acid (aa) sequence identity with mouse Smoothelin.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04895 is reactive to SMTN in Human
Observed Molecular Weight
120 kDa
Calculated molecular weight
99.1 kDa
Background of SMTN
Smoothelin is a protein that in humans is encoded by the SMTN gene. This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04895 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human COLO320 whole cell, human SW620 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Smoothelin/SMTN using anti-Smoothelin/SMTN antibody (A04895).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothelin/SMTN antigen affinity purified polyclonal antibody (Catalog # A04895) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothelin/SMTN at approximately 120 kDa. The expected band size for Smoothelin/SMTN is at 99 kDa.
Specific Publications For Anti-Smoothelin/SMTN Antibody Picoband® (A04895)
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Customer Q&As
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4 Customer Q&As for Anti-Smoothelin/SMTN Antibody Picoband®
Question
Is this A04895 anti-Smoothelin/SMTN antibody reactive to the isotypes of SMTN?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The immunogen of A04895 anti-Smoothelin/SMTN antibody is A synthetic peptide corresponding to a sequence of human Smoothelin (QELEREEEALASKRFRAERQDNKENWLHSQQREAEQR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-15
Question
We want to test anti-Smoothelin/SMTN antibody A04895 on mouse fetus for research purposes, then I may be interested in using anti-Smoothelin/SMTN antibody A04895 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
The products we sell, including anti-Smoothelin/SMTN antibody A04895, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-10
Question
We are currently using anti-Smoothelin/SMTN antibody A04895 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
The anti-Smoothelin/SMTN antibody (A04895) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-08
Question
I was wanting to use your anti-Smoothelin/SMTN antibody for IF for mouse fetus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetus identification?
A. Jha
Verified customer
Asked: 2013-10-02
Answer
It shows on the product datasheet, A04895 anti-Smoothelin/SMTN antibody has been tested for IF, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-02
