Overview
| Product Name |
Anti-MEK1/MAP2K1 Antibody
See more MAP2K1 products |
| Catalog# |
PA1376 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for MEK1/MAP2K1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MEK1/MAP2K1 information: Molecular Weight: 43439 MW; Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis; Tissue Specificity: Widely expressed, with extremely low levels in brain. |
| Cite This Product |
Anti-MEK1/MAP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1376)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-MEK1/MAP2K1 Antibody may replace the following items: sc 101733|sc 135701|sc 136542|sc 219|sc 219 G|sc 219 X|sc 271914|sc 293106|sc 30635 R|sc 365800|sc 365800 X|sc 436|sc 6250|sc 7995|sc 7995 R|sc 81503|sc 81504. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Predicted Reactivity |
Hamster
*This antibody is predicted to react with the above species based on antigen
sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1(353-367aa KQLMVHAFIKRSDAE), identical to the related mouse and rat sequences. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For MAP2K1 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q02750 |
|---|
| NCBI Gene Id | 5604 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | MAP2K1 |
|---|
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 1 |
|---|
| Superfamily | protein kinase superfamily |
|---|
| Alternative Names | MAP2K1|dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) |
|---|
| Molecular Weight | 43439 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MAP2K1, check out the MAP2K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for MAP2K1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the MAP2K1 infographic
Our Boster Quality Guarantee for Anti-MEK1/MAP2K1 Antibody covers its use in the following applications.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-MEK1 antibody, PA1376, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: COLO20 Cell Lysate
Anti-MEK1 antibody, PA1376, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-MEK1 antibody, PA1376, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-MEK1 antibody, PA1376, ICC
ICC: MCF-7 Cell
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Questions and answers from customers related to PA1376 Anti-MEK1/MAP2K1 Antibody
1 Related Questions
Question
We are currently using anti-MEK1/MAP2K1 antibody PA1376 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
P. Krishna
Asked: 2014-12-05
Answer
The anti-MEK1/MAP2K1 antibody (PA1376) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-05
