Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 1(MAP2K1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Anti-MEK1/MAP2K1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever (FHF) is caused by defects in TNFRSF1A gene.
|Product Name||Anti-MEK1/MAP2K1 Antibody
See all MAP2K1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MEK1/MAP2K1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MEK1/MAP2K1 information: Molecular Weight: 43439 MW; Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis; Tissue Specificity: Widely expressed, with extremely low levels in brain.|
|Cite This Product||Anti-MEK1/MAP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1376)|
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|Anti-MEK1/MAP2K1 Antibody may replace the following items: sc 101733|sc 135701|sc 136542|sc 219|sc 219 G|sc 219 X|sc 271914|sc 293106|sc 30635 R|sc 365800|sc 365800 X|sc 436|sc 6250|sc 7995|sc 7995 R|sc 81503|sc 81504.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1(353-367aa KQLMVHAFIKRSDAE), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MAP2K1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5604|
|Species Of This Entry||Human|
|Protein Name||Dual specificity mitogen-activated protein kinase kinase 1|
|Superfamily||protein kinase superfamily|
|Alternative Names||MAP2K1|dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 18.104.22.168; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Ubiquitination; Acetylation; Oxidation; Biotinylation; Reduction; Farnesylation; Geranylgeranylation; Myristoylation; Prenylation; Ribosylation; Deacetylation; Gamma-carboxylation; Methylation|
|Subcellular Localization||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Membrane; Peripheral membrane protein. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742).|
|Gene Location||15q22.31, on Chromasome 15, gene sequence: NC_000015.10 (66386912..66491544)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MAP2K1 (Source: Uniprot.org, NCBI)
|Protein Function||Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.|
|Tissue Specificity||Widely expressed, with extremely low levels in brain.|
|Interacting Proteins||BANP; BIRC6; BRAF; BTRC; MAPK1; MAPK3; PIN1; RAF1; VRK2; YAP1|
|Research Areas||Angiogenesis; Autophagy; Cancer; MAP Kinase Signaling; Phospho-Specific; Protein Phosphatase; Signal Transduction; Tyrosine Kinases|
|Related Diseases||Malignant Neoplasms; Neoplasms; Cell Transformation, Neoplastic; Carcinoma; Inflammation; Leukemia; Tissue Adhesions; Mammary Neoplasms; Malignant Neoplasm Of Breast; Cell Invasion; Melanoma; Hyperplasia; Neoplasm Metastasis; Malignant Paraganglionic Neoplasm; Neoplasm Invasiveness; Tumor Angiogenesis; Cardio-facio-cutaneous Syndrome; Malignant Neoplasm Of Lung; Congenital Heart Defects; Lung Neoplasms|
|Related Pathways||Cell Proliferation; Cell Cycle; Cell Death; Cell Growth; Secretion; Localization; Pathogenesis; Mapk Cascade; Angiogenesis; Cell Migration; Cell Cycle Arrest; Cell Differentiation; Induction Of Apoptosis; Cytokine Production; Mitosis; Cell Activation; S Phase; Rna Interference; Transport; Senescence|
|Background||Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.|
|Scientific References||PMID: 1281467 by Seger R., et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases.|
PMID: 8388392 by Zheng C.-F., et al. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2.
Our Boster Quality Guarantee for Anti-MEK1/MAP2K1 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: COLO20 Cell Lysate
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
ICC: MCF-7 Cell
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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