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Product Info Summary
| SKU: | PA1376 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-MEK1/MAP2K1 Antibody
SKU/Catalog Number
PA1376
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK1/MAP2K1 Antibody catalog # PA1376. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK1/MAP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1376)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1376 is reactive to MAP2K1 in Human, Mouse, Rat
Applications
PA1376 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
43.439kDa
Background of MAP2K1
Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al. (1997) localized the MAP2K1 gene to 15q22.1-q22.33.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-MEK1 antibody, PA1376, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: CEM Cell Lysate
Lane 4: COLO20 Cell Lysate
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Anti-MEK1 antibody, PA1376, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-MEK1 antibody, PA1376, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-MEK1 antibody, PA1376, ICC
ICC: MCF-7 Cell
Protein Target Info & Infographic
Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K1
Full Name
Dual specificity mitogen-activated protein kinase kinase 1
Weight
43.439kDa
Superfamily
protein kinase superfamily
Alternative Names
dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K1, check out the MAP2K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK1/MAP2K1 Antibody (PA1376)
Hello CJ!
No publications found for PA1376
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MEK1/MAP2K1 Antibody
Question
We are currently using anti-MEK1/MAP2K1 antibody PA1376 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
P. Krishna
Verified customer
Asked: 2014-12-05
Answer
The anti-MEK1/MAP2K1 antibody (PA1376) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-05
