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Product Info Summary
| SKU: | M01725-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8]
SKU/Catalog Number
M01725-2
Size
100 µl
Description
Boster Bio Anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI8A8 (formerly 8A8). Catalog# M01725-2. Tested in FC, IF, IHC, IP, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01725-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8A8
Isotype
IgG2b
Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector
Reactive Species
M01725-2 is reactive to MAP2K4 in Dog, Human, Mouse, Rat
Calculated molecular weight
44.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01725-2 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~1000
IHC 1:150
IF 1:50~100
Flow Cytometry 1:100
IP 2ug/500ul
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either MAP2K4 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (M01725-2)
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Anti-MAP2K4 mouse monoclonal antibody (M01725-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
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Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (M01725-2).
Specific Publications For Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8] (M01725-2)
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