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Product Info Summary
| SKU: | A05337 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Mena ENAH Antibody
SKU/Catalog Number
A05337
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Mena ENAH Antibody catalog # A05337. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mena ENAH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05337)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ENAH. AA range:472-521
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05337 is reactive to ENAH in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
66.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05337 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Mena Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from HepG2 cells, using ENAH Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ENAH Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Mena ENAH Antibody (A05337)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Mena ENAH Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Mena antibody A05337. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
Is a blocking peptide available for product anti-Mena antibody (A05337)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We do provide the blocking peptide for product anti-Mena antibody (A05337). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-24
Question
Is there a BSA free version of anti-Mena antibody A05337 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mena antibody A05337 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
We are currently using anti-Mena antibody A05337 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-Mena antibody (A05337) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-Mena antibody A05337 work for WB with liver?
K. Parker
Verified customer
Asked: 2018-08-31
Answer
According to the expression profile of liver, ENAH is highly expressed in liver. So, it is likely that anti-Mena antibody A05337 will work for WB with liver.
Boster Scientific Support
Answered: 2018-08-31
