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Product Info Summary
| SKU: | A03851-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Claudin 7/CLDN-7 Antibody Picoband®
SKU/Catalog Number
A03851-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Claudin 7/CLDN-7 Antibody Picoband® catalog # A03851-2. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 7/CLDN-7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03851-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Claudin 7/CLDN-7, which shares 87% and 100% amino acid (aa) sequence identity with human and rat Claudin 7/CLDN-7, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03851-2 is reactive to Cldn7 in Mouse
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.4 kDa
Background of Cldn7
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed constitutively in the mammary epithelium throughout development, and might be involved in vesicle trafficking to the basolateral membrane. It is essential for NaCl homeostasis in distal nephrons. The knockout mice lacking this gene showed severe salt wasting, chronic dehydration, and growth retardation, and died within 12 days after birth. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03851-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Positive Control
WB: mouse small intestine tissuer>
Validation Images & Assay Conditions
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Western blot analysis of Claudin 7/CLDN-7 using anti-Claudin 7/CLDN-7 antibody (A03851-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates.
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 7/CLDN-7 antigen affinity purified polyclonal antibody (Catalog # A03851-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 7/CLDN-7 at approximately 22KD. The expected band size for Claudin 7/CLDN-7 is at 22KD.
Specific Publications For Anti-Claudin 7/CLDN-7 Antibody Picoband® (A03851-2)
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