Anti-Galectin 1/Lgals1 Antibody Picoband™

Boster Bio Anti-Galectin 1/Lgals1 Antibody Picoband™ catalog # A00470. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00470
Size: 100ug/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Galectin 1/Lgals1 Antibody Picoband™

See all Galectin-1 products

SKU/Catalog Number

A00470

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 1/Lgals1 Antibody Picoband™ catalog # A00470. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 1/Lgals1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00470)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00470 is reactive to Lgals1 in Mouse, Rat

Applications

A00470 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Lgals1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09382

Gene ID

3956

Gene Name

Lgals1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lgals1, check out the Lgals1 Infographic

Lgals1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lgals1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00470 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ye Z,Huang S,Zhang Y,Mei X,Zheng H,Li M,Chen J,Lu F.Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model.Front Immunol.2020 Mar 13;11:146.doi:10.3389/fimmu.2020.00146.PMID:32231658;
Species: Mouse
A00470 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN AND INTESTINE TISSUE, DILUTION:1:500

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4 Customer Q&As for Anti-Galectin 1/Lgals1 Antibody Picoband™

Question

My colleagues were content with the WB result of your anti-Galectin 1/Lgals1 antibody. However we have been able to see positive staining in placenta skin extracellular space using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-12

Answer

From literature, placenta skin does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-04-12

Question

We ordered your anti-Galectin 1/Lgals1 antibody for WB on liver in a previous project. I am using mouse, and We are going to use the antibody for IHC next. I am interested in examining liver as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2017-10-30

Answer

I have checked the website and datasheets of our anti-Galectin 1/Lgals1 antibody and I see that A00470 has been validated on mouse in both WB and IHC. Thus A00470 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-10-30

Question

We have seen staining in rat lung fibroblast. Any tips? Is anti-Galectin 1/Lgals1 antibody supposed to stain lung fibroblast positively?

A. Bhatt

Verified customer

Asked: 2015-12-11

Answer

Based on literature lung fibroblast does express LGALS1. Based on Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-12-11

Question

We are currently using anti-Galectin 1/Lgals1 antibody A00470 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?

D. Singh

Verified customer

Asked: 2015-04-01

Answer

The anti-Galectin 1/Lgals1 antibody (A00470) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-04-01

Conjugation

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A00470
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