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Product Info Summary
| SKU: | RP1089 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Macrophage metalloelastase MMP12 Antibody Picoband®
SKU/Catalog Number
RP1089
PB0712 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Macrophage metalloelastase MMP12 Antibody catalog # RP1089. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrophage metalloelastase MMP12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1089)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse MMP12, different from the related human sequence by thirteen amino acids, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1089 is reactive to Mmp12 in Mouse
Observed Molecular Weight
55 kDa
Calculated molecular weight
55.0 kDa
Background of Mmp12
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1089 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: HEPA Whole Cell
Validation Images & Assay Conditions
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Anti-RP1089 antibody, PBMMP12, Western blotting
All lanes: Anti MMP12 (RP1089) at 0.5ug/ml
WB: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Specific Publications For Anti-Macrophage metalloelastase MMP12 Antibody Picoband® (RP1089)
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5 Customer Q&As for Anti-Macrophage metalloelastase MMP12 Antibody Picoband®
Question
We are currently using anti-MMP12 antibody RP1089 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The anti-MMP12 antibody (RP1089) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-06
Question
We bought anti-MMP12 antibody for ELISA on amniotic fluid in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. you antibody examining amniotic fluid as well as alveolar macrophage in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
R. Baker
Verified customer
Asked: 2019-11-29
Answer
I looked at the website and datasheets of our anti-MMP12 antibody and it seems that RP1089 has been validated on mouse in both ELISA and WB. Thus RP1089 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-29
Question
I am looking for using your anti-MMP12 antibody for negative regulation of endothelial cell-matrix adhesion via fibronectin studies. Has this antibody been tested with western blotting on hepa whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
I appreciate your inquiry. This RP1089 anti-MMP12 antibody is tested on hepa whole cell lysate. It is guaranteed to work for ELISA, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-11
Question
We have observed staining in mouse alveolar macrophage. Are there any suggestions? Is anti-MMP12 antibody supposed to stain alveolar macrophage positively?
B. Gonzalez
Verified customer
Asked: 2016-09-28
Answer
From literature alveolar macrophage does express MMP12. From Uniprot.org, MMP12 is expressed in amniotic fluid, alveolar macrophage, esophagus, among other tissues. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-09-28
Question
Our team were well pleased with the WB result of your anti-MMP12 antibody. However we have observed positive staining in amniotic fluid extracellular space using this antibody. Is that expected? Could you tell me where is MMP12 supposed to be expressed?
E. Thomas
Verified customer
Asked: 2013-10-10
Answer
According to literature, amniotic fluid does express MMP12. Generally MMP12 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-10-10
