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MMP9 antibody (PB9668) performs well in ELISA, IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 61 Publications

pb9668 mmp9 primary antibodies ihc testing 2

IHC analysis of MMP9 using anti-MMP9 antibody (PB9668).
MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-MMP9 Antibody Picoband®

MMP9 antibody

61 Publications

Reactive Species	Host	Application
Human	Rabbit	ELISA, IHC, WB

Tested Applications

MMP9 antibody was positively detected by WB in:

human A549 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MMP9 antibody was positively detected by IHC in:

human liver cancer tissue, human liver cancer tissue, human lymphadenoma tissue, human lymph nodes of gastric adenocarcinoma rectal cancer tissue, human colonic adenocarcinoma tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-MMP9 Antibody Picoband®
View all MMP9 Antibodies

Form

Lyophilized

Uniprot

P14780

Gene ID

4318

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9, different from the related mouse sequence by fourteen amino acids, and from the related rat sequence by sixteen amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

79 kDa

Calculated Molecular Weight

78.5 kDa

Background of MMP9

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Cite This Product

Anti-MMP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9668)

Protocols

Western Blot protocol for MMP9 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MMP9 antibody

Review Protocol

Publications

Sex differences in kidney and lung status in an animal model of brain death

Published: 2025-03-26

Journal: Clinics

Application: IHC

Impact Factor: 2.40

Species: Rat

PMID: 40147183

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Free Secondary Antibody

Anti-MMP9 Antibody Picoband®

MMP9 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Sex differences in kidney and lung status in an animal model of brain death

A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma

The expression and role of SUZ12 in lung adenocarcinoma

miR-769-3p inhibits cellular proliferation of KSHV-infected SH-SY5Y cells through targeting mTOR

The involvement of MALAT1-ALKBH5 signaling axis into proliferation and metastasis of human papillomavirus-positive cervical cancer

Microfibril-associated protein 2 is activated by POU class 2 homeobox 1 and promotes tumor growth and metastasis in tongue squamous cell carcinoma

LINC01635, a long non‑coding RNA with a cancer/testis expression pattern, promotes lung cancer progression by sponging miR‑455‑5p

MiR-135a-5p suppresses trophoblast proliferative, migratory, invasive, and angiogenic activity in the context of unexplained spontaneous abortion

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway

miR-34a-5p inhibits the malignant progression of KSHV-infected SH-SY5Y cells by targeting c-fos

Hops extract and xanthohumol ameliorate bone loss induced by iron overload via activating Akt/GSK3β/Nrf2 pathway

Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity

Combination of ferulic acid, ligustrazine and tetrahydropalmatine inhibits invasion and metastasis through MMP/TIMP signaling in endometriosis

Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance

Insulin-like growth factor-binding protein 3 inhibits angiotensin II-induced aortic smooth muscle cell phenotypic switch and matrix metalloproteinase expression

LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability

ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803

Suppressive activities of mangiferin on human epithelial ovarian cancer

Antitumor evaluation of carboxymethyl chitosan based norcantharidin conjugates against gastric cancer as novel polymer therapeutics

RRM2 promotes the progression of human glioblastoma

Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model

Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathway in Vitro and in Vivo

Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9

Effects of baicalein on proliferation, apoptosis, migration and invasion of Ewing's sarcoma cells

Kallikrein-related peptidase 4 contributes to the tumor metastasis of oral squamous cell carcinoma

PAD1 promotes epithelial-mesenchymal transition and metastasis in triple-negative breast cancer cells by regulating MEK1-ERK1/2-MMP2 signaling

Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma

RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma

Cytotoxic necrotizing factor 1 promotes prostate cancer progression through activating the Cdc42–PAK1 axis

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation

In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis

Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Growth differentiation factor 15 induces growth and metastasis of human liver cancer stem-like cells via AKT/GSK-3β/β-catenin signaling

Circular RNA 0000096 affects cell growth and migration in gastric cancer

Gremlin1 promotes carcinogenesis of glioma in vitro

Silencing of ATPase family AAA domain-containing protein 2 inhibits migration and invasion of colorectal cancer cells.

TGF-β1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

SPOCK1 promotes the proliferation, migration and invasion of glioma cells through PI3K/AKT and Wnt/β-catenin signaling pathways

NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation Corrigendum in /10.3892/mmr.2016.4976

Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo

Preparation and anti-tumor metastasis of carboxymethyl chitosan

Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line

miR-494-3p Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by PTEN/AKT Signaling in Human Glioblastoma Cells

Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway

Correlations of lysyl oxidase with MMP2/MMP9 expression and its prognostic value in non-small cell lung cancer

Expression and correlation of matrix metalloproteinase-9 and heparanase in patients with breast cancer

Clinical significance of integrin β6 as a tumor recurrence factor in follicular thyroid carcinoma

Effect of siRNA targeting MTA1 on metastasis malignant phenotype of ovarian cancer A2780 cells

Expression and Correlation Analysis of β-adrenoceptors, VEGF, MMP-9 and Caspase-3 in Different Phases of Infantile Hemangioma

Influences of the interferon induced transmembrane protein 1 on the proliferation, invasion, and metastasis of the colorectal cancer SW480 cell lines

Effect of human cytomegalovirus on invasive capability of early pregnant extravillous cytotrophoblasts

Expression of VEGF and MMP-9 and MRI imaging changes in cerebral glioma

In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function

Hypoxia activates heparanase expression in an NF-κB dependent manner

RhoC expression level is correlated with the clinicopathological characteristics of ovarian cancer and the expression levels of ROCK-I, VEGF, and MMP9

Profiling and comparing transcription factors activated in non-metastatic and metastatic nasopharyngeal carcinoma cells

Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Expression of MMP-9 and TIMP-1 in lesions of systemic sclerosis and its implications

Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro

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