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Product Info Summary
| SKU: | PA1498 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IGFBP3 Antibody Picoband®
SKU/Catalog Number
PA1498
BA3641 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
IGFBP3 binds IGF-I/IGF-II and supports formation of the IGF:IGFBP3:ALS circulating complex, modulating IGF transport and bioavailability (and IGF-independent signaling is often discussed in the literature; putative unless experimentally established in your system). Assay context: Human/Rat-reactive antibody validated for Western blot; commonly interpreted with upstream protease activity (e.g., ECM-protease programs such as MMP3) and with immune-infiltration readouts (e.g., myeloid markers like CD163) when IGF signaling is studied in inflamed or tumor tissues (putative pairing).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1498)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IGFBP-3.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1498 is reactive to IGFBP3 in Human, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
31.7 kDa
Background of IGFBP3
IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1498 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: 293T Whole Cell, MCF-7 Whole Cell, A549 Whole Cell, SW620 Whole Cell, rat liver tissue, rat kidney tissue, rat heart tissue
Validation Images & Assay Conditions
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Anti-IGFBP-3 antibody, PA1498, Western blotting
All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 40KD
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Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (PA1498).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # PA1498) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40KD. The expected band size for IGFBP3 is at 32KD.
Specific Publications For Anti-IGFBP3 Antibody Picoband® (PA1498)
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1 Customer Q&As for Anti-IGFBP3 Antibody Picoband®
Question
Can PA1498 detect mouse IGFBP3?
Verified customer
Asked: 2019-04-25
Answer
The WB and IHC test result on mouse samples are not good. We do not recommend using the Anti-IGFBP3 Antibody (PA1498) on mouse samples.
Boster Scientific Support
Answered: 2019-05-08
