Anti-Laminin LAMA1 Monoclonal Antibody

Product Name

Anti-Laminin LAMA1 Monoclonal Antibody

View all Lama1 Antibodies

SKU/Catalog Number

MA1054

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Laminin LAMA1 Monoclonal Antibody catalog # MA1054. Tested in IHC applications. This antibody reacts with Human, Pig.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Laminin LAMA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1054)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

LAM-89

Isotype

Mouse IgG1

Immunogen

Human laminin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1054 is reactive to Lama1 in Human, Pig

Applications

MA1054 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

338.148kDa

Background of Lama1

Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, feline, pig, By Heat

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Laminin using anti-Laminin antibody (MA1054).
Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Laminin Antibody (MA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For Lama1 (Source: Uniprot.org, NCBI)

Alternative Names

LAMA; Laminin A chain; laminin subunit alpha-1; laminin, alpha 1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-LAM alpha; S-laminin subunit alpha Lama1|AA408497, L, Lama|laminin, alpha 1|laminin subunit alpha-1|S-LAM alpha|S-laminin subunit alpha|laminin A chain|laminin-1 subunit alpha|laminin-3 subunit alpha

For more info on Lama1, check out the Lama1 Infographic

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In this infographic, you will see the following information for Lama1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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