|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Morg1 Antibody|
|Description||Rabbit IgG polyclonal antibody for WD repeat domain-containing protein 83(WDR83) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Morg1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1053)|
|Replacement Item||This antibody may replace the following items: sc-74680|sc-74681 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Morg1(29-43aa RAVRFNVDGNYCLTC), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||WD repeat domain-containing protein 83|
|Molecular Weight||34343 MW|
|Protein Function||Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). May also be involved in pre-mRNA splicing. .|
|Sequence Similarities||Belongs to the WD repeat MORG1 family.|
|Subcellular Localization||Cytoplasm . Nucleus . Predominantly cytoplasmic. Partially nuclear. .|
|Alternative Names||WD repeat domain-containing protein 83;Mitogen-activated protein kinase organizer 1;MAPK organizer 1;WDR83;MORG1;|
|Research Areas|||cardiovascular|hypoxia|associated proteins| signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| epigenetics and nuclear signaling|dna / rna|rna processing|splicing|cardiovascular/immune|prolyl hydroxylase||
Background for WD repeat domain-containing protein 83
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Morg1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Morg1 Antibody Images
Click the images to enlarge.
WB: Rat Brain Tissue Lysate
IHC(P): Rat Lung Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,