Rat CXADR ELISA Kit PicoKine™

Rat CXADR ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cxadr in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1726
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Rat CXADR ELISA Kit PicoKine™

See all Cxadr products

SKU/Catalog Number

EK1726

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat CXADR ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cxadr in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat CXADR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1726)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G237

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1726 is reactive to Cxadr in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1726 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1726-CAP Anti-Rat Cxadr Pre-coated 96-well strip microplate 1
EK1726-ST Rat Cxadr Standard 2 vials, 10 ng/tube
EK1726-DA Rat Cxadr Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0370.1320.2280.3750.6911.1831.9222.267

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cxadr, Dilution ratio of 1:1, concentration in serum is around 65pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat CXADR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5924580861249932
Standard deviation3.611.5245.253.918.272.7
CV (%)6.1%4.7%5.6%6.4%7.3%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cxadr in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 159546060582.484.2%
Sample 224520421421722015.216.9%
Sample 380892492283987350.945.8%
*number of samples for each test n=16.

Gene/Protein Information For Cxadr (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9R066

Gene ID

89843

Gene Name

Cxadr

Full Name

Coxsackievirus and adenovirus receptor homolog

Weight

39.949kDa

Alternative Names

CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR Cxadr|Car1|CXADR, Ig-like cell adhesion molecule|coxsackievirus and adenovirus receptor homolog|CAR|coxsackie virus and adenovirus receptor|rCAR

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cxadr, check out the Cxadr Infographic

Cxadr infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cxadr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Rat CXADR ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have obtained CXADR level in serum.

Verified Customer

Verified customer

Asked: 2020-06-30

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-06-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Huang

Verified customer

Asked: 2019-10-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-10-23

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-09-20

Question

Q: how can I thaw whole blood sample for CXADR ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXADR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-01

Question

Q: if the enzyme conjugated CXADR antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXADR antigen?

Verified Customer

Verified customer

Asked: 2018-07-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-07-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-01-25

Question

Q: can I use citrate plasma as samples in Rat CXADR Picokine® ELISA Kit (Catalog # EK1726)?

Verified Customer

Verified customer

Asked: 2017-12-06

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CXADR causing degradation of its protein structure. CXADR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXADR ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-12-06

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Z. Rodriguez

Verified customer

Asked: 2015-07-23

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-07-23

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