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Product Info Summary
|Application:||Flow Cytometry, WB|
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Anti-MSH2 Monoclonal Antibody
Boster Bio Anti-MSH2 Monoclonal Antibody catalog # M00140. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MSH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00140)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human MSH2 MSH2 (MutS homologue 2) forms the hMutS- Alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS- Alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00140 is reactive to MSH2 in Human
M00140 is guaranteed for Flow Cytometry, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 FC 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MSH2 expression in HeLa cell lysate using anti-MSH2 antibody (M00140).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # M00140) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MSH2.
Gene/Protein Information For MSH2 (Source: Uniprot.Org, NCBI)
DNA mismatch repair protein Msh2
DNA mismatch repair MutS family
COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1); LCFS2; MSH2; mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli); MutS protein homolog 2 MSH2 COCA1, FCC1, HNPCC, HNPCC1, LCFS2, MMRCS2, hMSH2 mutS homolog 2 DNA mismatch repair protein Msh2|DNA mismatch repair protein Msh2 transcript|mutS homolog 2, colon cancer, nonpolyposis type 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MSH2, check out the MSH2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MSH2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M00140
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