Anti-MSH2 Monoclonal Antibody

Boster Bio Anti-MSH2 Monoclonal Antibody catalog # M00140. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00140
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-MSH2 Monoclonal Antibody

See all MSH2 products

SKU/Catalog Number

M00140

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MSH2 Monoclonal Antibody catalog # M00140. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MSH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00140)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACIH-13

Immunogen

A synthesized peptide derived from human MSH2 MSH2 (MutS homologue 2) forms the hMutS- Alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS- Alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00140 is reactive to MSH2 in Human

Applications

M00140 is guaranteed for Flow Cytometry, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000 FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For MSH2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P43246

Gene ID

4436

Gene Name

MSH2

Full Name

DNA mismatch repair protein Msh2

Weight

104.743kDa

Superfamily

DNA mismatch repair MutS family

Alternative Names

COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1); LCFS2; MSH2; mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli); MutS protein homolog 2 MSH2 COCA1, FCC1, HNPCC, HNPCC1, LCFS2, MMRCS2, hMSH2 mutS homolog 2 DNA mismatch repair protein Msh2|DNA mismatch repair protein Msh2 transcript|mutS homolog 2, colon cancer, nonpolyposis type 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MSH2, check out the MSH2 Infographic

MSH2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MSH2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M00140

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14 Customer Q&As for Anti-MSH2 Monoclonal Antibody

Question

I was wanting to use your anti-MSH2 Monoclonal antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

It shows on the product datasheet, M00140 anti-MSH2 Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-31

Question

I have a question about product M00140, anti-MSH2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00140 anti-MSH2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-14

Question

you antibody to test anti-MSH2 Monoclonal antibody M00140 on human blood for research purposes, then I may be interested in using anti-MSH2 Monoclonal antibody M00140 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

The products we sell, including anti-MSH2 Monoclonal antibody M00140, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-05

Question

Here is the WB image, lot number and protocol we used for blood using anti-MSH2 Monoclonal antibody M00140. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-28

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-MSH2 Monoclonal antibody M00140. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-12

Question

Is a blocking peptide available for product anti-MSH2 Monoclonal antibody (M00140)?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

We do provide the blocking peptide for product anti-MSH2 Monoclonal antibody (M00140). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-30

Question

Is this M00140 anti-MSH2 Monoclonal antibody reactive to the isotypes of MSH2?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

The immunogen of M00140 anti-MSH2 Monoclonal antibody is A synthesized peptide derived from human MSH2 MSH2 (MutS homologue 2) forms the hMutS- Alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS- Alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-26

Question

Is there a BSA free version of anti-MSH2 Monoclonal antibody M00140 available?

T. Jones

Verified customer

Asked: 2019-08-21

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MSH2 Monoclonal antibody M00140 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-21

Question

Does anti-MSH2 Monoclonal antibody M00140 work for WB with blood?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

According to the expression profile of blood, MSH2 is highly expressed in blood. So, it is likely that anti-MSH2 Monoclonal antibody M00140 will work for WB with blood.

Boster Scientific Support

Answered: 2019-05-21

Question

We are currently using anti-MSH2 Monoclonal antibody M00140 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

S. Yang

Verified customer

Asked: 2018-08-17

Answer

The anti-MSH2 Monoclonal antibody (M00140) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-17

Question

My team were well pleased with the WB result of your anti-MSH2 Monoclonal antibody. However we have been able to see positive staining in uterus nucleus. using this antibody. Is that expected? Could you tell me where is MSH2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-13

Answer

From literature, uterus does express MSH2. Generally MSH2 expresses in nucleus. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-02-13

Question

Would M00140 anti-MSH2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-07

Answer

You can see on the product datasheet, M00140 anti-MSH2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-07

Question

We have seen staining in human muscle. Are there any suggestions? Is anti-MSH2 Monoclonal antibody supposed to stain muscle positively?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

Based on literature muscle does express MSH2. Based on Uniprot.org, MSH2 is expressed in secondary oocyte, uterus, testis, muscle, blood, embryonic kidney, erythroleukemia, among other tissues. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-07-27

Question

I see that the anti-MSH2 Monoclonal antibody M00140 works with WB, what is the protocol used to produce the result images on the product page?

N. Mitchell

Verified customer

Asked: 2014-03-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-03-17

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00140
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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