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Product Info Summary
| SKU: | M00140-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375
SKU/Catalog Number
M00140-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375 (Catalog # M00140-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00140-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM375
Isotype
Rabbit IgG
Immunogen
A mammalian cells expressed recombinant protein fragment within 500 amino acids of the N-terminus of human Msh2
Cross-reactivity
This antibody reacts to human DNA mismatch repair protein Msh2
Reactive Species
M00140-1 is reactive to MSH2 in Human
Calculated molecular weight
104.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00140-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:1000 dilution
WB: 1:400-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of HeLa lysate using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:500 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:100 dilution.
Specific Publications For Anti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375 (M00140-1)
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Customer Q&As
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8 Customer Q&As for Anti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375
Question
We have observed staining in human testis. Any tips? Is anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Based on literature testis does express MSH2. Based on Uniprot.org, MSH2 is expressed in secondary oocyte, uterus, testis, muscle, blood, embryonic kidney, erythroleukemia, among other tissues. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-24
Question
Is this M00140-1 anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 reactive to the isotypes of MSH2?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The immunogen of M00140-1 anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 is A mammalian cells expressed recombinant protein fragment within 500 amino acids of the N-terminus of human Msh2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-04
Question
We are currently using anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 M00140-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 (M00140-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
Our team were happy with the WB result of your anti-MSH2 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
From literature, embryonic kidney does express MSH2. Generally MSH2 expresses in nucleus. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-13
Question
I was wanting to use your anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
As indicated on the product datasheet, M00140-1 anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
My lab would like to test anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 M00140-1 on human testis for research purposes, then I may be interested in using anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 M00140-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Taylor
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 M00140-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
We bought anti-MSH2 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
I have checked the website and datasheets of our anti-MSH2 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-03-06
Question
Our lab want to know about using your anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 for double-strand break repair studies. Has this antibody been tested with western blotting on colon cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
We appreciate your inquiry. This M00140-1 anti-MSH2 Rabbit Monoclonal antibody, Clone#RM375 is tested on colon cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-23
